摘要
建立柱前衍生-反相高效液相色谱,同时测定黄酒中17种氨基酸及7种生物胺含量的方法。采用异硫氰酸苯酯柱前衍生试剂,采用Inertsil ODS-SP C_(18)(250 mm×4.6 mm,5μm)分析,以乙酸钠溶液和乙腈溶液混合液进行梯度洗脱,在254 nm波长处进行氨基酸和生物胺的含量分析。氨基酸和生物胺在2.0~500 mg/L和0.5~125 mg/L范围内具有良好的线性关系,相关系数R^2大于0.99,平均回收率在86.44%~99.75%之间,相对标准偏差在0.31%~3.53%之间;检出限为0.02~0.25 mg/L,定量限为0.25~4.50 mg/L。方法线性范围广、准确性高、稳定性好,适用于黄酒中氨基酸及生物胺的检测。
A reversed-phase high performance liquid chromatographic（RP-HPLC）method has been presented for the simultaneous determination of 17 free amino acids and 7 biogenic amines in Chinese rice wine.Wine samples were precolumn derivatized with phenylisothiocyanate（PITC）,separated using Inertsil ODS-SP C_（18） chromatography column（250 mm ×4.6 mm,5 μm）,eluted with a mobile phase composed of sodium acetate,acetonitrile and ultra-pure water,and detected at 254 nm.The calibration curves of 17 amino acids and 7 biogenic amines all exhibited good linearity over the range from 2.0 to 500 mg/L and 0.5 to 125 mg/L,respectively.Mean recoveries for amino acids and biogenic amines were between 86.44%and 99.75%,and the relative standard deviations（RSDs）were in the range of 0.31%–3.53%.The limits of detection（LODs）for 17 free amino acids and 7 biogenic amines were in the range of 0.02–0.25 mg/L,and the limits of quantification（LOQs）were 0.25–4.50 mg/L.The method is stable,accurate,and suitable for the simultaneous determination of amino acids and biogenic amines in Chinese rice wine,having a wide linear range.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第8期159-163,共5页
Food Science
基金
绍兴市重点科研项目(2014A22002)
关键词
黄酒
氨基酸
生物胺
反相高效液相色谱
Chinese wice wine
amino acids
biogenic amines
reversed-phase high performance liquid chromatography（RP-HPLC）
