摘要
建立高效液相色谱法同时测定工夫红茶中咖啡碱、儿茶素、表儿茶素、表没食子儿茶素、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯、茶黄素、茶黄素-3-没食子酸酯、茶黄素-3’-没食子酸酯、茶黄素双没食子酸酯10种内含物成分含量的方法。通过色谱条件优化,确定出工夫红茶中10种内含物成分含量测定的最佳色谱条件为：色谱柱为Whatman Partisphere C_（18）液相色谱柱（4.6 mm×125 mm,5μm）、柱温32℃、流动相为0.035%三氟乙酸-5%乙腈溶液和0.025%三氟乙酸-50%乙腈溶液、流速1 m L/min、进样量10μL、检测波长205 nm。在此条件下,上述10种内含物成分得到很好的分离和测定。各种内含物的线性范围为0.05~500μg/m L,标准曲线相关系数均在0.999 8以上,精密度检测相对标准偏差为0.72%~3.32%（n=5）,重复性检测相对标准偏差为1.87%~4.43%（n=5）,回收率在94.73%~102.38%之间。该方法简单、快捷、准确、重复性好,可应用于工夫红茶中10种内含物质的同时快速测定。
A high performance liquid chromatographic（HPLC）method was established for the simultaneous determination of ten components including caffeine,catechin（C）,epicatechin（EC）,epigallocatechin（EGC）,epicatechin gallate（ECG）,epigallocatechin gallate（EGCG）,theaflavin（TF1）,theaflavin-3-gallate（TF2A）,theaflavin-3＇-gallate（TF2B）,theaflavin-3,3＇-digallate（TF3）,in Congou black tea.The chromatographic conditions were optimized.A Whatman Partisphere C18column（4.6 mm × 125 mm,5 μm）was used to separate the ten compounds.The mobile phase was a mixture of 0.035%trifluoroacetic acid containing 5% acetonitrile（A）and 50% acetonitrile solution containing 0.025% trifluoroacetic acid and methanol solution（B）at a flow rate of 1 m L/min.The column temperature and UV detection wavelength were set as 32 ℃and 205 nm,respectively.The ten components were successfully separated and detected under the chosen experimental conditions.The calibration curves for all these components displayed a good linear relationship with correlation coefficients above 0.999 8 and precision relative standard deviations（RSDs）of 0.72%–3.32%（n = 5）and repeatability RSD of 1.87%–4.43%（n = 5）.The recovery rates of the ten compounds were in the range of 94.73%–102.38%.The method is convenient,rapid,accurate,reproducible and applicable to simultaneously determine these ten components in Congou black tea.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第8期97-101,共5页
Food Science
基金
"十二五"国家科技支撑计划项目(2012BAF07B05-4)
关键词
工夫红茶
高效液相色谱法
内含物
测定
Congou black tea
high performance liquid chromatographic（HPLC）
components
determination
