摘要
黄大茶是中国特有的茶叶品种,具有降脂、降糖、改善代谢综合征的保健功效,但由于缺乏对黄大茶化学成分的研究,其功效性物质基础尚未被完全揭示。本研究以超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q TOF/MS)作为检测工具,结合中性丢失和特征碎片离子等信息,对黄大茶的主要化学成分进行筛查和分析。选取Waters ACQUITY UPLC BEH C18色谱柱(100 mm×21 mm,17μm)进行分离,以01%甲酸水和乙腈作为流动相进行梯度洗脱,流速02 mL/min,进样量2μL,柱温35℃。在电喷雾电离(ESI)正/负离子模式下,利用全信息串联质谱(MSE)技术采集黄大茶样品溶液的质谱信息。通过查阅文献构建茶叶化学成分数据库,主要包括化学名称、分子式、结构式、准分子离子、碎片离子等信息,对自建数据库中的化学成分按照骨架结构进行归类;基于UPLC-Q TOF/MS测定结果,利用对照品对主要类别化合物的质谱裂解规律进行梳理,并总结其特征碎片离子和中性丢失特征;结合化合物的保留时间、准分子离子、碎片离子等信息对化合物结构进行表征。本研究共从黄大茶中鉴定出87个化学成分,包括10个儿茶素类、32个黄酮类、16个酚酸类、12个鞣质类、6个茶黄素类以及11个其他类别化合物,其中14个成分经对照品予以验证。该方法能够全面阐明黄大茶的主要化学成分,为黄大茶功效性成分发现、品质评价提供科学依据和数据支撑。
Large-leaf yellow tea,a slightly fermented yellow tea that is unique to China,has a stronger hypoglycemic effect than other tea varieties,such as green and black tea.Research on large-leaf yellow tea has focused on its hypoglycemic effect owing to the lack of comprehensive techniques to characterize its chemical components;thus,its development and further promo-tion are limited.Therefore,the development of a reliable analytical method to fully characterize the chemical components of large-leaf yellow tea is urgently required.In this study,a reliable strategy based on the data-acquisition technology of ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q TOF/MS)was established to rapidly screen and analyze the main chemical components of large-leaf yellow tea by combining the in-formation of neutral loss groups and characteristic fragment ions.The chromatographic separa-tion experiments were performed on a Waters ACQUITY UPLC BEH C18 column(100 mm×21 mm,17μm)with gradient elution using 01%formic acid aqueous solution and acetonitrile as the mobile phases.The flow rate was 02 mL/min,the sample volume was 2μL,and the col-umn temperature was 35℃.The mass spectral information of the components in a large-leaf yellow tea solution was collected using the full-information tandem MS(MSE)technique in pos-itive and negative ion modes.The specific chemical components of large-leaf yellow tea was identified as follows.First,a self-established database of tea chemical components was con-structed based on the literature.The mass spectral cleavage pathways of different types of com-pounds in large-leaf yellow tea were then sorted using reference substances,and the character-istics of the fragment ions and neutral loss groups were summarized.The precise mass-to-charge ratio of the target chemical components were then obtained based on the mass spectral information.Finally,the structures of the compounds in large-leaf yellow tea were confirmed based on their chromatographic retention time
作者
黄若童
戎雪雯
付晓洁
陈畅
储俊
许娜
吴欢
HUANG Ruotong;RONG Xuewen;FU Xiaojie;CHEN Chang;CHU Jun;XU Na;WU Huan(Research and Technology Center,Anhui University of Chinese Medicine,Hefei 230038,China;State Key Laboratory of Tea Plant Biology and Utilization,Anhui Agricultural University,Hefei 230036,China;Anhui Province Key Laboratory of Research and Development of Chinese Medicine,Hefei 230012,China;Functional Activity and Resource Utilization on Edible and Medicinal Fungi Joint Laboratory of Anhui Province,Hefei 230038,China)
出处
《色谱》
CAS
CSCD
北大核心
2024年第9期837-855,共19页
Chinese Journal of Chromatography
基金
国家自然科学基金(81903765)
安徽农业大学茶树生物学与资源利用国家重点实验室开放基金项目(SKLTOF20220105).
关键词
超高效液相色谱-四极杆-飞行时间质谱
中性丢失
特征碎片离子
黄大茶
化学成分
ultra performance liquid chromatography-quadrupole time-of-flight mass spectrom-etry(UPLC-Q TOF/MS)
neutral loss
characteristic fragment ions
large-leaf yellow tea
chemical components
