摘要
以园林绿化树种假连翘为试材,通过不同浓度的甜菜碱和氯化钙处理,测定在-3-6℃4个温度梯度下的叶片相对电导率、可溶性糖和脯氨酸含量、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性、氧自由基产生速率及过氧化氢（H2O2）含量,综合评价各个药剂处理对假连翘耐寒性的影响。结果表明：8个处理的相对电导率、脯氨酸含量、POD活性随温度的降低而呈现不同程度的上升趋势,可溶性糖含量也基本随温度的下降而上升;各处理的SOD活性变化呈先升高后下降的趋势;除-3℃外,各处理的氧自由基产生速率和过氧化氢含量也呈先上升后下降的变化趋势;与8个处理耐寒性关系密切的生理指标为电导率、脯氨酸含量和氧自由基产生速率,通过隶属函数法综合评判得出,20mmol/L氯化钙和20mmol/L甜菜碱＋10mmol/L氯化钙处理对提高假连翘耐寒性的效果较好,研究为园林绿化管养提供了一定的理论指导。
Taking the garden greening tree species Duranta repens as experimental material.Through different concentrations of betaine and calcium chloride treatment,relative conductance,the contents of soluble sugar and proline,the activities of superoxide dismutase（SOD）and peroxidase（POD）,oxygen free radical generation rate and hydrogen peroxide（H2O2）content were investigated on four different temperature conditions from-3℃ to 6℃.The results showed that the relative conductance,proline content,POD activity of eight treatments increased to varying degrees with the lowering of the temperature.Overall change trend of the soluble sugar content also increased.The SOD of all treatments activity increased first and then declined.In addition to-3℃,the oxygen free radical production rate and hydrogen peroxide content of each treatment also showed a trend of first increasing and then decreasing.The physiological indexes with close relation to the cold resistance of the eight treatments were relative conductance,proline content and oxygen free radical production rate.By the comprehensive evaluation with subordinate function,20 mmol/L calcium chloride and 20mmol/L betaine＋10mmol/L calcium chloride treatments had the better effect on cold resistance of Duranta repens and the study for garden plant conservation could provide a theoretical guidance.
出处
《北方园艺》
CAS
北大核心
2016年第8期154-159,共6页
Northern Horticulture
基金
国家自然科学基金资助项目(31300302)
云南省高校优势特色重点学科(生态学)建设资助项目
昆明学院引进人才科研资助项目(YJL11030)
关键词
假连翘
甜菜碱
氯化钙
低温胁迫
耐寒性
生理指标
Duranta repens
betaine
calcium chloride
low temperature stress
cold resistance
physiological index
