摘要
近年来,我国食品中砷(As)超标事件频发,严重威胁着我国人民的身体健康与生命安全,从而引起人们对砷危害性的高度重视。虽然传统的重金属检测技术能对食品中的As进行低浓度的测定,但是同时存在仪器昂贵、样品前处理复杂、检测成本较高、需专业人员进行操作等缺点,不利于食品中应急事件的快速检测,因此仍需要发展一种成本低廉、简单快捷、准确高效的检测方法,用于食品中重金属As的快速检测。生物传感器技术由于具有操作简单、检测成本低、选择性好、灵敏度高、分析速度快且能在复杂的体系中进行在线连续监测等特点,使生物传感器技术在食品安全领域中是一项具有长远意义的新技术,同时非常适用于食品中应急事件的快速检测。本文主要以大肠杆菌、蛋白质、DNA和核酸适配体为基础的生物传感器为例,对生物传感器技术的作用机理及在食品中重金属As检测中的最新应用进展作重点综述,并对生物传感器技术在食品安全领域的发展前景进行了展望。
In recent years, the events of arsenic contamination in foods arose frequently in China, which seriously threaten human health and life safety and have gained high attention due to the harmfulness of arsenic. Although the traditional analytical techniques enable detection of arsenic at low concentrations in foods, they have many shortcomings such as expensive instruments, sophisticated sample pretreatment, high cost and professional operation. Thus, there is still the necessity of developing a low-cost, simple and quick, accurate and efficient method for the detection of arsenic in foods. Due to its characteristics such as simple operation, low detection cost, good selectivity, high sensitivity, rapid analysis and continuous monitoring on-line in a complex system, biosensor technology is a new technology with long-term significance in the field of food safety, and it is very suitable for rapid detection of arsenic contamination in food safety incident emergency handling. In this article, the working mechanisms of biosensors based on E. coli, protein, DNA and aptamer as well as the latest progress in their application for the detection of arsenic in foods are reviewed. Moreover, future applications of biosensor technology in the field of food safety are also discussed.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第7期233-238,共6页
Food Science
基金
国家自然科学基金青年科学基金项目(31001065)
