摘要
基于水热法合成了形貌均一的纳米Co_3O_4,利用其强催化性能将其连同纳米Pt和辣根过氧化物酶(HRP)层层修饰于金电极表面,最终构建出对H_2O_2具有三重催化作用的新型纳米复合材料Co_3O_4-Pt-HRP修饰电极,并将其应用于对H_2O_2的浓度检测。实验结果发现,纳米Co_3O_4与纳米Pt的催化作用相互协同,并且纳米Pt较大的比表面积和优良的生物相容性增加了后续辣根过氧化物酶的负载量与生物催化活性,从而提高了传感器的灵敏度。在优化条件下,对H_2O_2浓度的检测范围为2.0μmol/L^7.1 mmol/L,检出限为0.9μmol/L。此外,该传感器还表现出较高的选择性、稳定性和灵敏度。
A sensitive triple signal amplifying electrochemical biosensor is prepared based on a novel horseradish peroxidase（ HRP） functionalized Co_3O_4-Pt nanocomposite for the detection of H_2O_2. The Co_3O_4nanoparticles（ Co_3O_4NPs） are successfully synthesized with hydrothermal method. The synergistic catalytic performance of Co_3O_4 and Pt nanoparticles is observed. Moreover,the large specific surface and excellent biocompatibility of Pt nanoparticles greatly enhanced the load and biology catalytic activity of HRP,increasing the sensitivity of the electrochemical biosensor. Under optimal experimental conditions,the dynamic range of the resulted biosensor for the detection of H_2O_2 is from 2. 0 μmol to 7. 1 mmol with a detection limit of 0. 9 μmol（S/N = _3）.
出处
《现代化工》
CAS
CSCD
北大核心
2016年第4期81-84,86,共5页
Modern Chemical Industry
基金
国家自然科学基金项目(21065009)
教育部重点项目(210251)
兵团重点领域创新团队计划(2015BD003)
