摘要
实验选取东北农业大学高、低脂双向选择系(NEAUHLF)第18世代的4周至7周龄肉鸡为实验材料,采用Real-Time RT-PCR方法检测鸡腹部脂肪组织中DNA甲基转移酶(DNMT1、DNMT3A和DNMT3B)的表达量;采用基因组甲基化检测试剂盒检测分析鸡腹部脂肪组织基因组DNA甲基化的差异。结果表明:低脂鸡腹部脂肪组织中的DNMT1和DNMT3A表达量均显著高于高脂鸡(P<0.05);除5周龄鸡之外,低脂鸡脂肪组织中DNMT3B的表达量也显著高于高脂鸡(P<0.05)。基因组DNA甲基化分析发现,低脂鸡腹部脂肪组织基因组DNA甲基化的总体水平高于高脂鸡(P<0.05)。结果提示,DNA甲基化调控鸡脂肪组织生长发育,DNA甲基化差异可能是高、低脂鸡腹脂性状差异的原因之一。
The fat and lean broilers of the 18 th generation of Northeast Agricultural University broiler lines divergently selected for abdominal fat content（NEAUHLF） from 4 to 7 weeks of age were used to detect the expression of DNA methyltransferases（DNMT1, DNMT3 A and DNMT3B） and global DNA methylation in the abdominal adipose tissues using real-time RT-PCR and Methyl FlashTMMethylated DNA Quantification Kit. The results showed that the expression levels of DNMT1 and DNMT3 A genes were significantly higher in the lean line than in the fat line（ P 〈0.05）. DNMT3 B gene expression was lowly expressed in chicken adipose tissue, comparatively, it was higher expressed in the lean line at 4, 6 and 7 weeks of age than in the fat line（ P 〈0.05）. Consistently, DNA methlyation analysis showed that the global DNA methylation levels in abdominal adipose tissue were significantly higher in lean line than in fat line in all tested ages（P 〈0.05）. Our results indicated that DNA methylationis involved in chicken adipose development and the DNA methylation differencemay contribute to the fatness trait difference in NEAUHLF.
出处
《中国畜牧杂志》
CAS
北大核心
2016年第7期22-26,共5页
Chinese Journal of Animal Science
基金
国家973计划课题(2009CB941604)
国家863课题(2011AA100301)
