摘要
目的调查本地腹泻病人和家畜家禽、昆虫苍蝇中小肠结肠炎耶尔森菌的感染及携带情况;通过对小肠结肠炎耶尔森菌的病原学和分子生物学研究来探讨动物带菌与人群之间的相互关系;为制定相应的防治对策提供科学依据。方法按照中国CDC编制《小肠结肠炎耶尔森菌实验室的病原学分离鉴定手册》第二、三版规定的程序进行病原学和分子生物学试验、研究。结果近年来自1 521份腹泻病人、家畜家禽等标本中分离出小肠结肠炎耶尔森菌109株,其阳性检出率分别为4.11%和11.04%;所有菌株均经生物梅里埃API 20E生化试验鉴定确认;不同标本中均检出致病性小肠结肠炎耶尔森菌,其毒力基因分布为ail+、ystA+、yadA+、virF+、rfbC+、ystB-,而非致病性菌株上述毒力因子均为阴性;致病性小肠结肠炎耶尔森菌PFGE分子分型检测证实腹泻病人,家畜家禽标本中的菌株带型类聚图高度一致。结论本地腹泻病人、家畜家禽和苍蝇标本均检出致病性和非致病性小肠结肠炎耶尔森菌;不同标本中分离的致病性小肠结肠炎耶尔森菌菌株毒力基因分布相同,均携带毒力质粒,PFGE分子分型检测基因组带型类聚图高度一致,从而可以认为猪是本地人群小肠结肠炎耶尔森菌病的重要传染源之一,牛、犬等在本病传播中的地位尚不能确定,有待进一步的调查与探讨。
We investigated the infection of Yersinia enterocoliticain local diarrhea patients,livestock and poultry,and insect flies carry status by studying relationship between host and animals carrier with etiological and molecular biological methods,for providing scientific basis for the development of appropriate countermeasures.Results showed that 109 Yersinia enterocolitica strains were isolated from 1 521 diarrhea patients and livestock poultry;the positive rates were 4.11% and11.04%,respectively.All strains were confirmed by bioMerieux API 20 Ebiochemical tests.Different specimens of pathogenic Yersinia enterocoliticastrains were detected,and the virulence genes include ail＋,ystA＋,yadA＋,virF＋,rfbC＋,ystB-,and pathogenic strains did not carry these virulence factors;pathogenic Yersinia enterocolitica PFGE subtyping tests showed that diagram with a highly consistent pattern clustering between diarrhea patients and host animals specimens.Pathogenic and non-pathogenic Yersinia enterocolitica were detected from local diarrhea patients,livestock poultry and fly specimens.Pathogenic Yersinia enterocolitica had the same virulence genes were isolated from different samples.Genome type clustering map were with a highly consistent pattern clustering tested by PFGE molecular,which prompted that swine were one of the most important sources of infection leading to the local people with Yersinia enterocolitica.It＇s also suggested that the effect of cattle and dogs cannot be determined in the spread of the disease,and needs further investigation and discussion.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2016年第3期256-261,共6页
Chinese Journal of Zoonoses
基金
国家科技重大专项课题(2013ZX10004-203-002)~~
关键词
小肠结肠炎耶尔森菌
腹泻病人
家畜家禽
分子生物学
Yersinia enterocolitica
diarrhea patients
livestock and poultry
molecular biology
