摘要
为研究小肠结肠炎耶尔森氏菌菌株GM2402对黄颡鱼的致病性及毒力基因分布,采用动物回归感染试验方法探讨菌株对黄颡鱼的致病性,利用PCR技术扩增毒力基因,通过药物敏感试验检测该菌株对抗生素的敏感性。在动物回归感染试验中,试验处理组鱼7d内的死亡率达100%,从感染后的试验鱼体中再次分离到小肠结肠炎耶尔森氏菌,证实了该菌株具较强致病性;成功扩增到粘附侵袭位点、耐热肠毒素、粘附素、毒力活化因子和强毒力岛上P4样噬菌体整合酶5种毒力基因,揭示毒力基因与致病性密切相关。菌株GM2402对卡拉霉素、恩诺沙星、磺胺异恶唑等9种药物高度敏感,对氟苯尼考、先锋霉素V中度敏感,而对链霉素、洁霉素等5种药物表现为耐药,这将为鱼类小肠结肠炎耶尔森菌病的有效防治提供参考。
The pathogenicity of bacterium Yersinia enterocolitica strain GM2402 isolated from yellow catfish （Pelteobagrus fulvidraco）was studied by artificial infection experiments, its virulence genes were amplificated by PCR, and the sensitivity of the pathogen to antibiotics was detected by a kirby-Bauer disk diffusion method. The artificial infection experiments showed that the healthy yellow catfish artificially infected with Y. enterocolitica isolated strain were all died in 7 days and then the pathogen was re-isolated from artificially infected fish, indicating that the pathogen had the pathogenicity. The main virulence factors including adhesion gene （aid, Yersinia stable heat-stable enterotoxin gene（ystB）, Yersinia adhesin gene（yadA）, transcriptional activator gene（virF） and P4-1ike integrase gene （intB） were successfully amplified, which were all involved in the pathogenicity. The Y. enterocolitica strain GM2402 as a pathogenic agent was found to be highly sensitive to compound sulfamethoxazole, kanamycin, gentamicin, tetracycline, enrofloxacin and the other four kinds of antibiotics, to be moderate sensitive to florfenicol, and cefalotin sodium, but resistant to streptomycin, furazolidone, cefaloridin lincomycin, and rocephin.
出处
《水产科学》
CAS
北大核心
2013年第5期293-296,共4页
Fisheries Science
基金
四川省科技厅应用基础项目(NO.2011JY0095)
西南民族大学研究生学位点建设项目(2011XWD-S071007)
