摘要
建立高效液相色谱-串联质谱仪测定婴幼儿食品中丙烯酰胺含量的方法。以13C3-丙烯酰胺为同位素内标,4%三氯乙酸水溶液作为提取液,经Na Cl盐析,乙腈萃取净化,通过高效液相色谱-串联质谱仪多反应监测模式进行测定,内标法定量。该方法的线性范围为12.24 ng/m L^612 ng/m L,相关系数r2>0.999 9,检出限为7μg/kg,定量限22μg/kg。3种婴幼儿食品及辅助食品中丙烯酰胺的加标回收率为96.5%~104.2%,相对标准偏差为0.9%~2.5%。本方法具有操作简便、准确率高、重现性好等优点,可应用于多种婴幼儿食品中丙烯酰胺含量的测定。
A method using isotope internal standard for determination acrylamide in infant foods had been established by ultra high-performance liquid chromatography coupled with quadrupole tandem mass spectrometry（HPLC-MS/MS）. The sample was extracted with 4 % trichloroacetic acid solution, and then purified by liquid-liquid extraction with acetonitrile, analyzed by HPLC-MS/MS in a multi-reaction monitoring（MRM） mode, an isotope internal standard was added for quantification. The correlation coefficient（r2） of acrylamide was more than 0. 999 9 in linear range of 12. 24 ng /m L-612 ng /m L. The detection limit and quantification limit were was 7 μg/kg and 22 μg/kg. The average spiked recoveries were in the range of 96.5 %-104.2 % with the relative standard deviations（RSD） of 0.9 %-2.5 %. The results showed this method was simple,high sensitivity, high accuracy and repeatable for the determination of acrylamide in kinds of infant foods.
出处
《食品研究与开发》
CAS
北大核心
2015年第24期134-137,共4页
Food Research and Development
基金
福建省质量技术监督局科技计划项目(FJQI2012057)
关键词
丙烯酰胺
超高效液相色谱-串联质谱法
婴幼儿食品
同位素内标
acrylamide
ultra performance liquid chromatography-tandem mass spectrometry
infant food
isotope internal standard
