摘要
同时测定中国不同产地甘草中18α-甘草酸和18β-甘草酸含量。采用高效液相色谱法,色谱柱EcosilC18分析柱,流动相为乙腈0.1%磷酸盐缓冲液（80∶20,p H=7）,体积流量为1.0 m L/min,检测波长为250 nm,柱温为30℃,进样量为10μL。18α-甘草酸和18β-甘草酸检测质量浓度分别在3.145-314.7、3.184-318.4μg/m L范围内与各自峰面积积分值呈良好的线性关（r=0.999 9）;精密度、稳定性、重复性试验的RSD≤1.03%;平均加样回收率分别为99.81%、99.47%,RSD分别为0.71%、0.94%（n=6）。内蒙古杭锦旗的甘草中甘草酸的含量较高,新疆喀什、宁夏盐池县出产的出产的甘草中甘草酸含量较接近。
Simultaneous determination of licorice 18α-and 18β-of glycyrrhizin licorice acid content of different Chinese origin by HPLC Column Ecosil-C18 analytical column; the mobile phase was acetonitrile 0. 1% phosphate buffer（ 80∶ 20,p H = 7）; the volumetric flow rate was 1. 0 m L / min; the detection wavelength was 250 nm; the column temperature was 30 ℃ and,injection amount was 10 μL.18α-and 18β-of glycyrrhizin licorice acid concentrations were detected in the 3. 145 - 314. 7μg / m L,3. 184 - 318. 4 μg / m L range.The respective peak area values showed a good linear relationship（ r = 0. 999 9）; precision,stability,RSD≤1. 03% repeatability test;the average recoveries were 99. 81%,99. 47%,and RSD was 0. 71%,0. 94%（ n = 6）. Inner Hangjinqi higher levels of glycyrrhizin in licorice,and Kashi Produced、Yanchi county produced glycyrrhizic acid content was closer.
出处
《药物生物技术》
CAS
2016年第1期55-57,共3页
Pharmaceutical Biotechnology
