摘要
本论文研究了新月弯孢霉KA-91对甘草次酸的微生物转化。该菌株在28℃、180 r/min条件下振荡培养24 h,加入终浓度为0.04%的甘草次酸乙醇溶液。56 h后,停止转化。通过TCL、HPLC、MS1、H NMR和13C NMR分析,转化产物鉴定为7β羟基甘草次酸,转化率为41.1%;该研究丰富了新月弯孢霉作用底物范围,为7β-羟基甘草次酸的合成提供了一条新途径。
The biotransformation of glycyrrhetinic acid by Curvularia lunataKA-91 was studied.After the strain was incubated for 24h at 28℃,180r/min,glycyrrhetinic acid was added to 0.04%（m/V）.The conversion reaction was ended after 56h.The purified product was identified as 7β-hydroxyl glycyrrhetinic acid by TCL,HPLC,MS,1H-NMR,and 13↑C-NMR techniques.The yield of 7β-hydroxyl glycyrrhetinic acid was 41.1% under the above biotransformation conditions.This result increased the substrate range of biotransformation by Curvularia lunata KA-91,and provided a novel method to the synthesis of 7β-hydroxyl glycyrrhetinic acid.
出处
《药物生物技术》
CAS
CSCD
2011年第3期238-241,共4页
Pharmaceutical Biotechnology
基金
天津市应用基础及前沿技术研究计划重点项目(08JCZDJC15200)
关键词
甘草次酸
新月弯孢霉
微生物转化
7β-羟基甘草次酸
Glycyrrhetinic acid
Curvularia lunata
Microbial transformation
7β-hydroxyl glycyrrhetinic acid
