摘要
目的:探讨纹状体腺苷A2A受体(A2AR)和多巴胺D2受体(D2DR)对大鼠力竭运动过程中苍白球胞外γ-氨基丁酸(GABA)与谷氨酸(Glu)释放的调控作用。方法:采用纹状体微量灌注和活体微透析-高效液相联用技术,实时、在线观察大鼠在一次力竭运动过程中苍白球细胞外液中GABA和Glu水平的动态变化规律,以及D2DR激动剂(喹吡罗,Quinpirole)和A2AR拮抗剂(SCH58261)对其影响,结合运动能力探讨D2DR和A2AR对疲劳的调控作用机制。结果:大鼠3级递增负荷跑台运动至力竭过程中,苍白球胞外Glu和GABA呈增高趋势,Glu/GABA呈降低趋势,在运动开始后45min至力竭各点Glu和GABA均显著高于安静水平(P<0.05),Glu/GABA显著低于安静水平(P<0.05),力竭后恢复90 min时Glu和GABA逐渐下降至安静水平,Glu/GABA逐渐恢复至安静水平;SCH58261和Quinpirole分别经纹状体灌注后,力竭运动过程中苍白球胞外Glu和GABA变化明显下降,大鼠自主运动期(疲劳初期)时间延长,大鼠跑台运动至力竭时间显著增加(P<0.05)。结论:在一次力竭运动过程中,大鼠苍白球神经元胞外Glu、GABA浓度及Glu/GABA均具有明显的阶段性特征,推测可能是力竭过程中大鼠运动能力呈现阶段性变化特征的机制之一;大鼠在力竭时,苍白球胞外Glu、GABA升高但Glu/GABA下降,推测力竭时大鼠苍白球神经元的兴奋性下降;SCH58261和Quinpirole经纹状体微量注射后,苍白球胞外Glu和GABA变化明显下降,大鼠自主运动期(疲劳初期)时间延长,大鼠跑台运动至力竭时间显著增加,推测可能是因为SCH58261和Quinpirole分别经各自作用机制干预了纹状体投向苍白球的GABA能传递而影响了间接通路对皮层的调控,最后达到延缓疲劳的作用。
Objective To explore the regulatory effect of striatal adenosine A2Areceptor（A2AR）and dopamine D2receptor（D2DR）on the extracellular gamma-aminobutyric acid（GABA）and glutamic acid（Glu）in globus pallidus during acute exhaustive exercise in rat. Methods Real-time and on-line dynamic variation of GABA and Glu in extracellular fluid in globus pallidus during the exercise and the effect of applying D2 DR agonist（quinpirole）and A2 AR antagonist（SCH58261）on the variation were observed using striatal microinfusion,microdialysis and high performance liquid chromatography technology. Results The Glu and GABA concentrations in globus pallidus increased and the ratio of Glu/GABA reduced during the exhaustive exercise. The Glu and GABA concentrations were both notably higher（P〈0.05）and the ratio of Glu/GABA was notably lower（P〈0.05）than that at rest from the beginning to 45 min after the exercise. The Glu and GABA concentrations decreased gradually and the ratio of Glu/GABA recovered gradually to the original level 90 min after the exercise. After the microperfusion of SCH58261 and quinpirole separately into striatum,the concentrations of extracellular Glu and GABA in globus pallidus decreased evidently,active movement stage prolonged,and time to exhaustion increased significantly（P〈0.05）. Conclusions Applying SCH58261 and quinpirole could delay the occurrence of exercise-induced fatigue,which was presumed that the striato-pallidal GABA neurotransmission was probably intervened.
出处
《中国运动医学杂志》
CAS
北大核心
2016年第3期248-256,共9页
Chinese Journal of Sports Medicine
基金
国家自然科学基金资助项目(31171138)
北京市自然科学基金资助项目(5142012)
关键词
运动性疲劳
纹状体
苍白球
腺苷A2A受体
多巴胺D2受体
exercise-induced fatigue
striatum
globus pallidus
adenosine A2Areceptor
dopamine D2 receptor
