摘要
【目的】对大叶相思花粉离体萌发适宜条件以及花粉活力不同检测方法进行研究,为今后进一步开展人工控制授粉、选育优良的马大杂交新品种提供基础。【方法】采回花穗的次日10:00之后,使用毛笔刷法收集大叶相思花粉;通过不同培养温度、不同蔗糖浓度和不同硼酸浓度处理后进行花粉离体萌发,筛选适宜大叶相思花粉离体萌发的条件;采用过氧化物酶法、I_2-KI法以及花粉管离体萌发法对大叶相思花粉进行活力测定,探讨大叶相思花粉有效的活力测定方法。【结果】大叶相思花粉在28℃下培养,其花粉平均萌发率为71.99%,花粉管平均长度为5.3 D(1 D=1倍的花粉粒长度),花粉管平均条数为6.2条,显著高于其他培养温度的处理。当培养基中含有200 g·L^(-1)蔗糖时,大叶相思花粉萌发率为84.96%,其花粉管平均长度为5.8 D,花粉管平均条数为6.2条,显著高于含有其他蔗糖浓度的处理。在含有300 mg·L^(-1)硼酸的培养基中培养大叶相思花粉,其平均萌发率为75.32%,花粉管平均长度可达4.8 D,平均花粉管条数为5.4条,显著高于在其他硼酸浓度下的处理。大叶相思花粉在培养温度30℃、200 g·L^(-1)蔗糖、300 mg·L^(-1)硼酸的培养基中萌发率为98.26%,花粉管长度均可达到10倍复合花粉粒的长度,最多可萌发出10条花粉管,显著高于其他处理。培养3 h的大叶相思花粉,萌发率为65.74%;培养6 h时,其萌发率为90.55%;培养24 h后,其萌发率趋于稳定,达到最高值98.26%。过氧化物酶法检测的花粉活力为99.67%,I_2-KI法检测的花粉活力为99.00%,花粉管离体培养法检测的花粉活力为98.15%,3种方法检测的花粉活力无显著差异。【结论】大叶相思花粉在不同的离体培养处理下,其萌发情况均不相同且差异显著,最佳离体萌发条件为200 g·L^(-1)蔗糖+300 mg·L^(-1)硼酸,培养温度为30℃,萌发率为98.26%。过氧化物酶法、I_2-KI法以及花
【Objective】This study was aimed to studying the suitable conditions of in vitro pollen germination and the methods of pollen viability test for Acacia auriculiformis in order to provide a basis for hybrid breeding of A. auriculiformis and creating new breeding material by artificial pollination. 【Methods 】Pollen of A. auriculiformis was collected after10： 00 am on the next day of collection of flower clusters using the brush method. The pollen was germinated in vitro in conditions with different temperatures,the different concentrations of sucrose and boric acid,and suitable conditions for in vitro pollen germination of A. auriculiformis were chosen. Peroxidase method,I_2-KI method and in vitro pollen germination method were used to detect the pollen viability of A. auriculiformis,and the effectiveness of these methods was assessed.【Results】When the pollen was cultured at 28 ℃,germination rate was 71. 99%,the average length of pollen tubes was5. 3 D（ 1D = the length of pollen grain）,the average number of pollen tubes was 6. 2,which were significantly higher than those of the other culture temperatures. When the culture medium contained 200 g · L^（-1）sucrose,the pollen germination rate was 84. 96%,the average length of pollen tubes was 5. 8 D,the average number of pollen tubes was 6. 2,which were significantly higher than those of the other sucrose concentrations. When the culture medium contained 300 mg·L^（-1）boric acid,the pollen germination rate was 75. 32%,the average length of pollen tubes could reach 4. 8 D,the average number of pollen tube was 5. 4,which were significantly higher than those of the other boric acid concentrations.A. auriculiformis pollen in the culture medium at 30 ℃ culture temperature with 200 g·L^（-1）sucrose and 300 mg·L^（-1）boric acid had a germination rate of 98. 26%,and the length of pollen tubes could be up to 10 times of the composite pollen length,and 10 pollen tubes could be produced at most,significantly higher than other treatments. When
出处
《林业科学》
EI
CAS
CSCD
北大核心
2016年第2期67-73,共7页
Scientia Silvae Sinicae
基金
国家"十二五"科技支撑计划项目(2012BAD01B0402)
关键词
大叶相思
花粉收集
花粉离体萌发
花粉活力测定
Acacia auriculiformis
pollen collection
in vitro pollen germination
pollen viability test
