摘要
目的探讨MicroRNA-139-5p(mi R-139-5p)在多型性胶质母细胞瘤中对细胞增殖和细胞周期的影响及其机制。方法通过miR-139-5p类似物、miR-139-5p抑制物和阴性对照microRNA分别转染U87MG和T98G细胞,RT-PCR分析miR-139-5p表达,通过MTT法观察其对细胞增殖的影响,通过流式细胞术检测细胞周期和凋亡,通过Western blot检测ELTD1蛋白表达。结果转染miR-139-5p后胶质瘤细胞增殖能力下降、细胞凋亡增加,细胞侵袭能力下降,流式细胞术结果显示S期细胞比例增加,G0/G1期细胞比例下降。Western blot分析显示在miR-139-5p过表达的细胞中,ELTD1蛋白水平显著下降。结论 miR-139-5p过表达可抑制胶质瘤细胞增殖、诱导细胞凋亡,其机制可能与下调ELTD1蛋白和调节细胞周期有关。
Objective To study the effect, molecular mechanisms and direct target gene of microRNA-139-5p（miR-139- 5p） on cell cycle and proliferation in glioblastoma multiform （GBM）. Methods MiR-139-5p overexpression or knock-down were established by transfecting miR-139-5p-mimics or miR-139-Sp-inhibitors into U87MG and T98G cells, and the effect on cell proliferation was studied by using MTY assay. The effect of miR-139-5p on cell cycle and apoptosis were measured by using flow cytometry assay. The expression of ELTD1 was discovered by Western blot assay. Results Glioma cells transfected with miR-139-Sp overexpression suppressed cell proliferation and increased cell apoptosis. The overexpression of miR-139-5p increased S phase decreased G0/G1 phase and inhibited ELTD1 expression in glioma cells. Conclusion Ectopic expression of miR-139-Sp in GBM cells significantly suppressed cell proliferation, regulated cell cycle and induced apoptosis by targeting ELTD1.
出处
《临床医学研究与实践》
2016年第3期1-3,24,共4页
Clinical Research and Practice
