摘要
目的:采用新型载体PEN对ODN进行传递,探讨MT01/PEN复合物对人成骨样细胞(MG63)细胞周期及成骨分化的影响。方法:选取不同装载比例的MT01/PEN复合物,以MT01及S-MT01做对照,分别检测其对MG63细胞周期及成骨分化相关基因ALP活性、BMP2、OCN表达的影响。结果:MT01/PEN组Gl期、G2期细胞百分比降低,S期比例增高(P<0.01),碱性磷酸酶活性增加(P<0.01),OCN、BMP2蛋白表达增高(P<0.05)。结论:以PEN为载体传递MT01能促进MG63的分化。
Objective：To adopt a new vector PEN-loading ODN and evaluate its effects on the cell cycle and osteogenic differentiation of osteoblastic cells.Methods：Different ratios of MT01/PEN complex were transfected into MG63 cells,while MT01 and S-MT01 were negative controls.After that,the cell cycle,ALP activity,and the expression levels of BMP2 and OCN were detected.Results：In MT01/PEN group,cell percentages in Gl and G2 phase were decreased,while that in S phase was increased（P〈0.01）.Meanwhile,ALP activity was increased（P〈0.01）,and the expression of OCN and BMP2 were significantly up-regulated（P〈0.05）.Conclusion：The application of PEN as a carrier of ODN could promote the osteogenic differentiation of MG63 cells.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2016年第3期301-304,共4页
Journal of Oral Science Research
