摘要
从山西省吕梁市郊区某养禽场送检的病死乌鸡中分离到1株新城疫病毒(Newcastle disease virus,NDV)。经血凝(HA)和血凝抑制(HI)试验鉴定,该分离株具有血凝性,血凝效价为2~9,且可被NDV阳性血清抑制,而不能被禽流感(H5、H9亚型)阳性血清所抑制。试验通过RT-PCR扩增了分离毒株F基因高度变异区362 bp的核苷酸序列。序列分析表明,该毒株含有1个开放阅读框(ORF),编码88个氨基酸;F蛋白裂解位点的氨基酸序列为^(112)R-R-Q-K-R-F^(117),符合强毒株裂解位点特征;同源性分析表明,分离毒株与典型毒株—LaSota、Clone30、F_(48)E_9、V_4及云南分离株KMLY-YM的核苷酸同源性为83.7%~86.5%,与山东分离株P914039S101同源性最高,达96.8%;遗传进化分析表明:分离毒株与山东分离株P914039S101处于同一进化分支内,属基因Ⅶd亚型。
One Newcastle disease virus （NDV） strain was isolated from silkie Chicken of poultry farm in LtiLiang, Shanxi Province. The results of the hemagglutination test（HA） and hemagglutina- tion inhabition test（HI） indicated that the isolated strain possessed Characteristic of hemagglutina- don, and Hemagglutination titer was 29. The HA of isolated strain can be inhibited by NDV positive serum, meanwhile, can＇t be inhibited by AIV （H5 subtype and H9 subtypes） positive serum. A 362 bp sequence of highly variable region in F gene of isolated strain was amplified by RT-PCR and cloned. The sequence analysis showed that the amplified sequence included 10RF and encoded 88 amino acids. Amino acid sequence of cleavage site in F protein was ll2R-R-Q-K-R-Fll7, which conformed with characteristic of virulent strain. Homology analysis indicated that the nucleotide homologies of isolated strain were 83.7% to 86.5%, compared with strain LaSota, strain Clone30, strain F48E9, strain V4 and strain KMLY-YM, and homology compared with strain P914039S101 was supreme, which was up to 96.8%. The phylogenetic analysis suggested that the isolated strain and strain P914039S101 were at same phylogenetie branch, and both belonged to genotype VIId.
出处
《经济动物学报》
CAS
2016年第1期13-17,共5页
Journal of Economic Animal
基金
国家星火计划项目(2014GA630001)
山西省科技攻关项目(20130311026-1)
山西省农业科学院畜牧兽医研究所级课题(XMS1001)
关键词
乌鸡
新城疫病毒
F基因
序列分析
silkie
newcastle disease virus
F gene
sequence analysis
