摘要
目的:观察妇科养荣胶囊(Fu Ke Yang Rong capsule,FKYRC)对卵巢损伤小鼠卵巢储备功能和生育力的保护作用。方法:通过成年雌性小鼠一次性腹腔注射120 mg/kg环磷酰胺和10 mg/kg白消安建立卵巢损伤小鼠模型,于造模前7 d至造模后60 d每日对卵巢损伤小鼠用高、中、低剂量(6 g/kg、4 g/kg和2 g/kg)FKYRC(H组、M组、L组)连续灌胃给药;同时,于造模前7 d一次性皮下注射给予Gn RHa(38 mg/kg)作为阳性对照组(Gn RHa组),用生理盐水(0.2 m L/d)代替FKYRC连续灌胃作为卵巢损伤对照组(模型组),另设正常对照组:非卵巢损伤正常小鼠腹腔注射给予等体积DMSO(NC组)。分别于造模后30 d和60 d取材,计数卵巢组织中各级卵泡数量,检测血清E2、FSH、抑制素B(INHB)和抗苗勒氏管激素(AMH)水平,检测卵巢组织中翼状螺旋/叉头转录因子2(FOXL2)和AMH蛋白的表达水平。并观察造模60 d后各组妊娠率和窝仔数。结果:各给药组和模型组小鼠血清E2低于NC组(P<0.05),但FSH水平组间无统计学差异(P>0.05)。造模各组卵泡数明显低于NC组(P<0.05),且H组和Gn RHa组造模后60 d时卵巢组织中窦前卵泡与窦卵泡数较高,但各干预组与模型组比较无统计学差异(P>0.05)。模型组小鼠卵巢组织中FOXL2和AMH蛋白表达水平显著低于各FKYRC干预组(P<0.05),H组的妊娠率(80.00%)显著高于模型组(36.36%)(P<0.05)。结论:连续FKYRC(6 g/kg)给药60 d后可明显改善烷化剂所致卵巢损伤小鼠的卵巢储备功能和生育能力,其机制可能与上调颗粒细胞中FOXL2和AMH的表达水平相关。
Objective： To study the protection of the FuKe YangRong capsule （FKYRC） on ovarian reserve function and fertility in ovarian injured mice. Methods： Totally 120 mg/kg cyclophosphamide and 10 mg/kg busulfan were administrated to 6-7-week-old C57 mice through intraperitoneal injection to induce ovarian injured model, and the normal control （NC） group were administerated with solvent dimethylsulfoxide （DMSO）. Positive group （GnRHa group） was administerated with GnRHa analogue triptorelin 38 mg/kg by using alone hypodermic injection before 7 d cyclophosphamide and busulfan treatment. The group of FKYRC received intragastric administration to mice from 7 d before cyclophosphamide and busulfan treatment to 60 d after cyclophosphamide and busulfan treatment, and the dosages were 6 g/kg （high-dose, H group）, 4 g/kg （medium-dose, M group） and 2 g/kg （low- dose, L group）, respectively. The ovarian injured control group were intragastric administrated with 0.2 mL saline （model group）. The ovaries were collected at 30 d and 60 d after cyclophosphamide and busulfan treatment, and the ovarian follicle was counted, serum E2, FSH and anti-Miillerian hormone （AMH） levels were detected by ELISA,the localization and expression of winged helix/forkhead transcrip-tion factor 2 （FOXL2） and AMH protein were checked by immunohistochemistry and Western blotting. The pregnant rate and litter size were analyzed at 60 d after cyclophosphamide and busulfan treatment. Results： The serum E2 was lower in all cyclophosphamide and busulfan treatment groups than in NC group （P〈0.05）, but FSH level was not significantly different among all groups. The number of ovarian follicle in all cyclophosphamide and busulfan treatment groups was lower than that in NC group （P〈0.05）, although the preantral follicle and antral follicle in GnRHa group and H group were higher, no significant difference was observed compared with other groups. The expressions of FOXL2 and AMH protein in model group were lowe
出处
《生殖与避孕》
CAS
CSCD
北大核心
2016年第3期161-170,共10页
Reproduction and Contraception
