摘要
目的观察微小RNA-1246(mi R-1246)在肝癌组织中的表达及其对人肝癌细胞增殖和凋亡的影响,探讨其在肝癌发生发展中的作用和意义。方法应用实时定量PCR(q RT-PCR)方法检测mi R-1246在肝癌以及癌旁组织的表达;对人肝癌细胞株(BEL7402、SMMC7721)转染mi R-1246抑制剂后,采用MTT法观察肝癌细胞的增殖活性,流式细胞仪检测细胞凋亡的变化。结果 mi R-1246在肝癌组织中表达显著高于癌旁组织[(65.39±8.77)vs(10.23±2.58),P=0.002]。MTT和凋亡实验结果显示,与阴性对照组相比,转染mi R-1246抑制剂后,BEL7402细胞增殖能力下降,凋亡增加(P均<0.05);SMMC7721细胞也出现增殖能力下降,凋亡增加(P均<0.05)。结论 mi R-1246在肝癌组织中高表达,并且与肝癌细胞的增殖及凋亡有关,其表达的上调可能与肝癌的发生发展有关。
Objective To observe the expression of miR-1246 in human hepatocellular carcinoma tissues and its effect on cell proliferation and apoptosis. Methods The expression of miR-1246 in hepatocellular carcinoma tissues and normal liver tissues was detected by real-time PCR（qRT-PCR）. The cultured hepatocellular carcinoma cell line BEL7402 and SMMC7721 cells were transfected with miR-1246 inhibitor, the cell proliferation was detected by MTT assay and apoptosis was analyzed by flow cytometry. Results The expression of miR-1246 in hepatocellular carcinoma tissues was significantly higher than that in normal liver tissues [（65.39±8.77） vs（10.23±2.58）, P=0.002）]. After transfected miR-1246 inhibitor, BEL7402 cell transfected hepatocellular carcinoma cells showed low proliferation rate and high apoptosis rate when compared with control group（P〈0.05）,the same trend was also observed in SMMC7721 cells（P〈0.05）. Conclusion These results indicate that the miR-1246 is highly expressed in hepatocellular carcinoma tissues and its high expression may be involved in the development of hepatocellular carcinoma.
出处
《肝胆胰外科杂志》
CAS
2016年第2期98-101,共4页
Journal of Hepatopancreatobiliary Surgery
基金
浙江省台州市科技项目(100KY-58)
关键词
微小RNA-1246
癌
肝细胞
增殖
凋亡
microRNA 1246（miR-1246）
hepatocellular carcinoma
proliferation
apoptosis
