摘要
目的:研究葛根素固体脂质纳米粒的制备工艺。方法:采用乳化-超声法制备葛根素固体脂质纳米粒,以单因素影响考察制备葛根素固体脂质纳米粒的工艺因素,通过正交试验设计优化制备工艺,并对包封率进行考察。结果:采用正交试验筛选出的最佳工艺为制备温度为78℃,加热时间为10min,搅拌速度为900rpm,超声时间为10min。根据筛选出的最佳工艺制备3批葛根素固体脂质纳米粒溶液,包封率为96.43%,载药量为1.47%。结论:通过工艺的筛选制备得到葛根素固体脂质纳米粒澄明度较好,有蓝色乳光,包封率和载药量较高,本研究方法可作为制备葛根素固体脂质纳米粒的制备方法。
Objective： To explore the preparation process of puerarin solid lipid nanoparticles. Methods： Puerarin solid lipid nanoparticles were prepared by using emulsion ultrasonic method. We investigated single factors in the preparation process. The optimum experimental process was filtrated by using orthogonal test. The drug loading of puerarin solid lipid nanoparticles were investigated in the research. Results： The optimum experimental process filtrated by using orthogonal test is that the preparation temperature is 78℃,the heating time is 10 min,the stirring speed is 900 rpm and the ultrasonic time is 10 min. According to the optimum experimental process,the 3 batches of puerarin solid lipid nanoparticles liquor are prepared in the research. The encapsulation rate is 96. 43%. The drug loading is 1. 47%. Conclusions： Puerarin solid lipid nanoparticles prepared by the selection of technology has preferable clarity,blue opalescence and higher encapsulation rate and drug loading. This method can be used to prepare the puerarin solid lipid nanoparticles.
出处
《黑龙江医药科学》
2016年第1期1-5,共5页
Heilongjiang Medicine and Pharmacy
基金
国家自然科学基金项目
编号:81274101
关键词
葛根素
固体脂质纳米粒
制备工艺
研究
puerarin
solid lipid nanoparticles
preparation technology
researc
