摘要
目的:研究大建中汤对脾阳虚胃癌大鼠模型环氧化酶-2(Cox-2)和核转录因子-кB(NF-кB)的影响。方法:取SD大鼠50只,随机分为5组,即空白对照组、模型对照组、大建中汤高剂量组、大建中汤低剂量组和西药对照组,每组10只。空白对照组给予生理盐水10 m L·kg^(-1),每天1次;模型对照组给予藩泻叶水浸液10 m L·kg^(-1),每天1次;大建中汤高剂量组、大建中汤低剂量组分别给予10.8 g·kg^(-1)和5.4g·kg^(-1),每天1次;西药对照组给予环磷酰胺2 m L/200 g,每天1次。各组灌胃15 d后处死,取出异常胃组织,甲醛溶液固定,石蜡包埋,切片。免疫组化检测各组Cox-2、NF-κB蛋白的表达。结果:与空白对照组比较,模型对照组大鼠胃组织Cox-2、NF-κB蛋白的含量明显升高(P<0.01);与模型对照组比较,大建中汤高剂量组、大建中汤低剂量组、西药对照组大鼠Cox-2、NF-κB蛋白的含量明显降低(P<0.01或P<0.05)。结论:大建中汤可调节胃组织Cox-2、NF-κB蛋白的水平,从而改善脾阳虚胃癌大鼠的症状。
Objective： To observe the influence of Major Center-Fortifying Decoction on Cox-2 and NF-кB of rats with gastric cancer of spleen-yang deficiency. Methods： Fifty SD rats were randomly divided into the following groups： the blank control group,the model control group,the high-dose Major Center-Fortifying Decoction group,the low-dose Major Center-Fortifying Decoction group and the Western medicine control group,with 10 cases in each group. The ones in the blank control group were given normal saline,10 m L·kg^（- 1）each time,once a day. The ones in the model control group were given francisco senna water extract,10 m L·kg^（- 1）each time,once a day. The ones in the high-dose Major Center-Fortifying Decoction group,and the low-dose Major CenterFortifying Decoction group were given 10. 8 g·kg^（- 1）and 5. 4 g·kg^（- 1）respectively each time,once a day. The ones in the Western medicine control group were given cyclophosphamide,2 m L /200 g each time,once a day. All the rats in the above groups were killed after 15 days intra-gastric administration. Their abnormal gastric tissues were taken out,and were fixed by formaldehyde solution to get section by paraffin imbedding. Then each group＇s Cox-2 and NF-кB protein expression were detected through immunohistochemistry method. Results： Compared with the blank control group,Cox-2 and NF-кB protein content of rats＇ gastric tissues in the model control group were obviously increased（ P 〈0. 01）. Compared with the model control group,Cox-2 and NF-кB protein content of rats＇ gastric tissues in high-dose Major Center-Fortifying Decoction group,the low-dose Major Center-Fortifying Decoction group and the Western medicine control group were obviously decreased（ P 〈0. 01 or P 〈0. 05）. Conclusion：Major Center-Fortifying Decoction can regulate Cox-2 and NF-кB protein level of gastric tissues,and improve the symptoms of rats with gastric cancer of spleen-yang deficiency.
出处
《河南中医》
2016年第2期227-229,共3页
Henan Traditional Chinese Medicine
基金
贵州省中医药管理局课题(编号:200712)
关键词
胃癌
脾阳虚
大建中汤
环氧化酶-2
核转录因子-КB
大鼠
Cancer of the stomach
gastric cancer of spleen-yang deficiency
Major Center-Fortifying Decoction
Cox-2
NF-кB
rat
