摘要
目的探讨趋化因子5（CCL5）及趋化因子受体5（CCR5）基因多态性与肺结核（pulmonarytuberculo—sis，PTB）易感性的关系。方法选取2014年2月至2015年4月首都医科大学附属北京胸科医院收治的PTB患者作为病例组，共494例；选取413名健康志愿者作为对照组。采集研究对象静脉血，使用竞争性等位基因特异性PCR方法检测其CCL5启动子区rs2107538和CCR5启动子区rs1799987基因型，计算病例组和对照组的基因型及等位基因频率，并对其分布情况进行比较，分析各基因多态性与PTB患病的相关性。结果CCL5启动子区rs2107538基因-403位点GG、GA、AA基因型及等位基因G、A的频次和频率病例组分别为：202（41％）、224（45％）、68（14％）和628（64％）、360（36％）；对照组分别为：152（38%）、209（51％）、52（13％）和513（62％）、313（38％），两组之间基因型分布和等位基因分布差异均无统计学意义（X2值分别为2．50和0．35，P值均〉0．05）。CCR5启动子区rs1799987基因-2459位点AA、AG、GG基因型及等位基因A、G的频次和频率病例组分别为：99（20％）、272（55％）、123（25％）和470（48％）、518（52％）；对照组分别为：67（16%）、210（51％）、136（33％）和344（42％）、482（58％），两组之间基因型分布和等位基因分布差异均有统计学意义（X2值分别为7．62和6．14，P值均〈0．05）。携带该位点基因型GO和等位基因G可降低PTB患病风险[OR（95％CI）值分别为：0．68（0．50～0．91）、0．78（0．64～0．94）]。结论CCL5启动子区rs2107538基因多态性与PTB患病无相关性；携带CCR5启动子区rs1799987基因-2459位点基因型GG和等位基因G可降低PTB患病风险。
Objective To study the relationship between CCL5, CCR5 gene polymorphism and susceptibility of pulmonary tuberculosis （PTB）. Methods A total of 494 PTB patients from Beijing Chest Hospital, Capital Medical University from February 2014 to April 2015 were selected as case group. Of 413 healthy volunteers were selected as a control group. By collecting venous blood, the single nucleotide polymorphisms （SNPs） of rs2107538 in CCL5 promoter region and rs1799987 in CCR5 promoter region were detected using competitive allele-specific PCR SNPline program. The genotype and allele frequencies for the SNPs were calculated for case group and control group separately in order to analyze the relationship between CCL5, CCR5 gene polymorphism and susceptibility of PTB. Results For the SNP of rs2107538 in CCL5, the frequencies for genotype GG, GA, AA and allele G, A were 202 （41%）, 224 （45%）, 68 （14%）, and 628 （64%）, 360 （36%） in case group; 152 （38%）, 209 （51%）, 52 （13%）, and 513 （62%）, 313 （38%） in control group. There was no statistically significant difference in genotype and allele frequencies between the two groups （X2= 2.50, 0. 35, all P values 〉0.05）. For the SNP of rs1799987 in CCR5, the genotype GG, GA, AA and allele G, A frequencies were 99 （20%）, 272 （55%）, 123 （25%） and 470 （48%）, 518 （52%） in case group; 67 （16%）, 210 （51%）, 136 （33%） and 344 （42%）, 482 （58%） in control group. The differences in genotype and allele frequencies between the two groups were statistically significant （X2 = 7. 62 for genotype frequencies, X2 =6.14 for allele frequencies, all P values 〈0. 05）. The C,C- genotype or G allele of rs1799987 in CCR5 may reduce the risk of PTB （ OR （ 95 % CI ） ： 0. 68 （ 0. 50 - 0. 91 ）, 0. 78 （ 0. 64 - 0. 94 ） ）. Conclusion There was no correlation between rs2107538 polymorphism in CCL5 and susceptibility of PTB. The GG genotype or G allele of rs1799987 in CCR5 promoter region may reduce the risk
出处
《中国防痨杂志》
CAS
2016年第3期193-197,共5页
Chinese Journal of Antituberculosis
关键词
趋化因子类
多态性
单核苷酸
结核
肺
疾病易感性
Chemotactic factors
Polymorphism, single nucleotide
Tuberculosis, pulmonary
Disease susceptibility
