摘要
目的：比较A7固体培养法和双相液体培养法在检测泌尿生殖道标本中解脲脲原体（Uu）和人型支原体（Mh）中的差异，并对两种方法学的结果进行分析比较，为实验室在判断结果时提供更多的参考价值。方法：把泌尿生殖道标本洗脱在双相液体培养基中，同时立即接种于A7固体培养基上，在含5％-10％C02的二氧化碳培养箱中36℃培养48小时后，A7培养基在10×低倍镜下倒置观察支原体特征性菌落及其他菌落，液体培养基观察液体颜色变化及液体特征。固体培养阴性的标本，从肉汤中取标本重新接种A7培养基继续培养后观察结果。结果：192例泌尿生殖道标本中，液体培养阳性为54例，阳性率为28％，A7培养基前后两次阳性分别为27例和35例，阳性率分别为14．06％和18．23％。结论：液体培养结果判断主观性强，提倡配套使用固体培养基，实验结果应以固体培养法为准。
Objective ： To Compare A7 solid culture method with dual-phase liquid culture method in the detection of Ureaplasma urealyticum（Uu） and Mycoplasma hominis （Mh） in different , and to analyze and compare the results of the two kinds of methods, for to provide the laboratory more reference value in judging the results. Methods ： Eluting the urogenital specimens in dual-phase liquid culture medium, and immediately inoculate on the A7 solid medium. After cultured for 48 hours in 5%-10%CO2 containing carbon dioxide incubator, observe Mycoplasma colony or other microbial at the A7 solid medium on low magnification inverted, and observe color changes and characteristics of liquid culture.Results ： In 192 cases of urogenital specimens, The number of positive cases of Liquid culture was 54, with the positive rate was 28%, Respectively, the two numbers of the positive cases of A7 culture medium was 27 cases and 35 cases, with the positive rate was14.06% and 18.23%. Conclusions ： The judgment of the liquid culture results is highly subjective, so advocating the use of solid culture medium together. The experimental results should be based on solid culture method as the standard.
出处
《医学检验与临床》
2016年第1期32-34,共3页
Medical Laboratory Science and Clinics
关键词
固体培养基
液体培养基
支原体
Sohd culture medium
Liquid culture medium
Mycoplasma
