摘要
目的探讨树舌灵芝提取物在实验动物体内对三阴乳腺癌的抑制作用。方法通过接种MDAMB-231-HM细胞建立三阴乳腺癌荷瘤小鼠模型,将20只造模成功的小鼠按随机数字表法分为树舌灵芝组和阴性对照组,每组10只,树舌灵芝组腹腔注射0.2 m L树舌灵芝水提物(100 mg/m L),阴性对照组给相同剂量的生理盐水,每3天用药1次,45天后停药。通过免疫组织化法检测CD34表达,以计数微血管;同时通过免疫组化法检测凝血酶敏感蛋白1(thrombospondin 1,TSP-1)及细胞周期蛋白D1(Cyclin D1)表达。结果树舌灵芝组小鼠瘤重[(0.33±0.16)g]明显低于阴性对照组[(0.68±0.37)g],两者差异有统计学意义(P<0.05);树舌灵芝提取物抑瘤率为51.4%。树舌灵芝组移植瘤体积明显小于阴性对照组,差异有统计学意义(P<0.05)。免疫组织化学染色结果显示:树舌灵芝组移植瘤中每个视野下微血管密度为(20.7±2.1)个、TSP-1阳性细胞数为(66.2±9.2)个、Cyclin D1阳性细胞数为(33.8±16.4)个;阴性对照组依次为(34.0±2.0)、(24.0±6.6)及(168.2±32.6)个。两组间比较,差异有统计学意义(P<0.05)。结论树舌灵芝提取物可能通过抑制肿瘤组织中血管的生成及调节细胞周期来抑制肿瘤细胞的过度增殖,从而实现对三阴乳腺癌的抑制作用。
Objective To study the inhibitory effect and mechanism of Ganoderma lipsiense extract（ GLE） on the growth of triple-negative breast cancer（ TNBC） cell line MDA-MB-231-HM in a mouse model. Methods The mouse model of TNBC was established by subcutaneous injection of 1. 5 × 106 of MDA-MB-231-HM cells into BALB / c-nu mouse. Twenty successfully modeled mice were divided into the GLE group and the negative control group according to random digit table,10 in each group. GLE（ 0. 2 m L 100 mg / m L） was peritoneally injected to mice in the GLE group,while equal dose of normal saline was peritoneally injected to mice in the negative control group. The medication was administered once per 3 days and discontinued after 45 days. The CD34 expression was detected using immunohistochemical assay for counting microvessels. Meanwhile,expressions of thrombospondin 1（ TSP-1） and cyclin D1 were detected using immunohistochemical assay.Results The average weight was obviously lower in the GLE group than in the negative control group [（ 0. 33 ± 0. 16） gvs（ 0. 68 ±0. 37） g,P 〈0. 05]. The tumor inhibition rate was 51. 4% in the GLE group. The volume of transplanted tumor was obviously lesser in the GLE group than in the negative control group（P 〈0. 05）. Results of immunohistochemical staining showed,the microvessel density（ MVD） under every field was（ 20. 7 ± 2. 1）,TSP-1 positive cell count was（ 66. 2 ±9. 2）,cyclin D1 positive cell count was（ 33. 8 ± 16. 4） in the GLE group,and they were 34. 0 ± 2. 0,24. 0 ± 6. 6,and168. 2 ± 32. 6,respectively in the negative control group. There was statistical difference in all indices between the two groups（P〈 0. 05）. Conclusion GLE could inhibit malignant proliferation of tumor cells by suppressing angiogenesis ofblood vessels in tumor tissues and regulating cell cycles,thereby inhibiting TNBC.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2016年第3期366-369,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
海南吉祥蕈野生灵芝真菌生物科技有限公司
关键词
树舌灵芝
三阴乳腺癌
抗肿瘤
Ganoderma lipsiense
triple-negative breast cancer
antineoplastic agent
