摘要
近年来,抗癌药的环境污染特征及其生态风险引起了广泛关注。为获取典型抗癌药5氟尿嘧啶的基础生态毒性数据,以蛋白核小球藻(Chlorella pyrenoidosa)和羊角月芽藻(Selenastrum capricornutum)为受试生物,考察了5氟尿嘧啶对2种绿藻的生长和叶绿素含量的影响。结果表明,5氟尿嘧啶对蛋白核小球藻和羊角月芽藻的生长具有抑制作用,随着暴露浓度升高,细胞生长抑制率增强。5氟尿嘧啶对2种绿藻的96 h半数抑制浓度(EC50)分别为450.36 mg·L^(-1)和692.30 mg·L^(-1),属于低毒性物质。暴露96 h后,低浓度5氟尿嘧啶(<32 mg·L^(-1))对蛋白核小球藻和羊角月芽藻叶绿素含量有一定的促进作用,高浓度5氟尿嘧啶(32~500 mg·L^(-1))则抑制了2种绿藻的叶绿素含量,且两者具有明显的负相关关系。和叶绿素b相比,叶绿素a对5氟尿嘧啶胁迫更为敏感。
The environmental occurrence and ecotoxicological risk of anticancer drugs（also called antineoplastic drugs） have attracted wide attentions. To obtain the basic ecotoxicology data of 5-fluorouracil, a typical anticancer drug, Chlorella pyrenoidosa and Selenastrum capricornutum were employed as target organisms, and effect of 5-fluorouracil on the growth and chlorophyll content of these two algae were examined. The results showed that thegrowth of C. pyrenoidosa and S. capricornutum were inhibited by 5-fluorouracil. The percentage of growth inhibition was enhanced with the increase of 5-fluorouracil concentration. The 96 h median effective concentration（EC50） of 5-fluorouracil to C. pyrenoidosa and S. capricornutum was 450.36 mg·L^（-1）and 692.30 mg·L^（-1）, respectively. 5-fluorouracil was low toxic to the two algae. After exposure to 5-fluorouracil for 96 h, higher chlorophyll content of C. pyrenoidosa and S. capricornutum was observed under low concentration of 5-fluorouracil（ 〈32 mg·L^（-1））, while high concentration of 5-fluorouracil（32-500 mg·L^（-1）） inhibited chlorophyll a and b content of C. pyrenoidosa and S. capricornutum with a negative correlation. Chlorophyll a is more sensitive to 5-fluorouracil.
出处
《生态毒理学报》
CAS
CSCD
北大核心
2015年第6期213-218,共6页
Asian Journal of Ecotoxicology
基金
江苏省高校自然科学研究面上项目(15KJB610006)
江苏高校优势学科建设工程资助项目(PAPD)
南京林业大学高层次人才基金项目(163010675)
关键词
抗癌药
5氟尿嘧啶
蛋白核小球藻
羊角月芽藻
生长
叶绿素
anticancer drugs
5-fluorouracil
Chlorella pyrenoidosa
Selenastrum capricornutum
growth
chlorophyll
