摘要
通过检测纤细裸藻生长、抗氧化酶活性和单细胞凝胶电泳(彗星试验)研究了4,4’-二溴联苯醚(BDE-15)对纤细裸藻(Euglenagracilis)的生态遗传毒性效应.结果表明,低浓度BDE-15(3×10-6mg/L)对纤细裸藻的生长无显著影响,高浓度时(3mg/L)具有明显的抑制作用,相比空白抑制率达69.70%;叶绿素a和类胡萝卜素含量在高浓度BDE-15作用下显著上升;谷胱甘肽(GSH)和细胞总蛋白含量则随BDE-15浓度增加明显下降;抗氧化酶系统中超氧化物歧化酶(SOD)活性随BDE-15浓度升高显著下降,具明显的剂量-效应关系,过氧化物酶(POD)活性随BDE-15浓度增加呈上升趋势,最高浓度组(3mg/L)比空白对照提高93.45%,显示BDE-15胁迫可诱导抗氧化酶活性;彗星试验结果显示纤细裸藻细胞DNA损伤程度随BDE-15浓度增加而加重,表明高浓度BDE-15具有潜在致突变性.
The potential ecogenotoxicities of 4, 4＇-dibromodiphenyl ether （BDE-15）, one of polybrominated diphenyl ethers （PBDEs） were investigated by detecting its effects on growth, pigment contents, antioxidant enzyme activities and DNA damage in microalgae Euglena gracilis. At lower concentrations （3×10^-6 mg/L）, BDE-15 showed no notable effect on the growth ofE. gracilis, while at higher concentration （3mg/L）, the growth was restrained to 69.70% compared with the control, meanwhile the content of photosynthetic pigments increased. Glutathione （GSH） and protein contents decreased with the increase of BDE-15 concentration. In addition, there was an obvious decrease and dose-effect relationship in SOD activity with the increase of BDE-15 concentration. At higher concentration （3mg/L）, POD activity increased by 93.45% compared with the control, which indicated that BDE-15 could induce the activities of antioxidant enzymes in E. gracilis. The degree of DNA damage observed from the comet assay increased with BDE-15 concentration increasing, which suggested that high dose of BDE-15 may have potential mutagenicity on E. gracilis.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2010年第6期833-838,共6页
China Environmental Science
基金
江苏省社会发展项目(BS2007049)
国家“973”项目(2008CB418003)
