摘要
目的:研究麦冬各提取物组对脂多糖诱导的巨噬细胞RAW264.7中炎症介质的影响,进而找出该药的抗炎有效部位群。方法:采用回流提取及有机溶剂萃取的方法制备麦冬总提取物及不同提取部位。各提取部位分别作用于RAW264.7巨噬细胞后,使用MTS法检测细胞活力;并对脂多糖诱导的RAW264.7细胞炎症反应模型采用不同部位的麦冬提取物和LPS(脂多糖)进行药物干预24 h后,采用Griess法测定NO的分泌量;采用ELISA法检测细胞上清液中TNF-α的含量。结果:麦冬各提取物组都能明显抑制LPS诱导的RAW264.7巨噬细胞NO的分泌(P<0.05),以及TNF-α的表达(P<0.01),并呈现出良好的剂量依赖性。与其他组相比,其中水提取部位对NO分泌的抑制作用最突出,正丁醇提取部位对TNF-α表达的抑制作用最突出。结论:麦冬各提取物组可通过抑制LPS诱导的RAW264.7巨噬细胞NO的分泌与TNF-α的表达,从而发挥抗炎作用,试验结果表明麦冬水与正丁醇提取部位对炎症因子的抑制作用最强。
Objective： To study the effect of different fractions extracted from Radix Ophiopogonis on inflammatory factors in LPS-induced RAW264. 7 cells and to find its anti-inflammatory parts. Methods： The methods of reflux extraction and organic solvent extraction were performed to obtain the fractions from the extract of Radix Ophiopogonis. After treating cells with different extracting fractions from Radix Ophiopogonis,the MTS assay was used to detect cell viability. The model of RAW264. 7 cell inflammatory reaction induced by Lipopolysaccharide was pretreated by different fractions extracted from Radix Ophiopogonis and LPS for 24 h. At last,the concentration of NO in supernatant was analyzed by Griess reaction,and the concentration of TNF-α in supernatant was analyzed by ELISA. Results： The concentration of NO and TNF-α was obviously inhibited by different fractions extracted from Radix Ophiopogonis and inflammatory factors in dose dependent manners. Particularly,the water extractive fraction had a greater inhibitory effect on NO secretion,and the n-butyl alcohol extractive fraction had a greater inhibitory effect on the expression of TNF-α. Conclusion： Different fractions of Radix Ophiopogonis have anti-inflammatory activities. The mechanism of the anti-inflammation of different fractions from Radix Ophiopogonis may be related to the inhibition of the release of NO and TNF-α from macrophages. The results indicate that the water and n-butyl alcohol extractive fractions have the best activities.
出处
《中医药信息》
2016年第2期11-15,共5页
Information on Traditional Chinese Medicine
关键词
麦冬
不同提取部位
RAW264.7细胞
炎症因子
Radix Ophiopogonis
Different extracted fractions
RAW264.7 cells
Inflammatory factors
