摘要
利用荧光光谱法研究了1,4,7-三氮杂环壬烷合钴(Ⅱ)与牛血清白蛋白的相互作用,实验结果表明1,4,7-三氮杂环壬烷合钴(Ⅱ)对牛血清白蛋白的荧光猝灭是静态猝灭,并得出在水溶液和胶束溶液中1,4,7-三氮杂环壬烷合钴(Ⅱ)与牛血清白蛋白作用的结合点位数、结合常数和热力学参数,△H>0,△S>0,其分子间的作用力主要表现为疏水作用力.
The interaction of 1,4,7-Triazacyclononan-Co（Ⅱ）（Cyclen-Co（Ⅱ））with bovine serum albumin（BSA）has been investigated by fluorescence method.The results show that the Cyclen-Co（Ⅱ）strongly quenched fluorescence of BSA.It was proved that the fluorescence quenching mechanism belonged to the static quenching by Stern-Volmer equation.According to Lineweaver-Burk equation and thermodynamics equation,the binding constants,binding sates and thermodynamics parameters were obtained at different temperatures in the aqueous solution and in the solutions of Brij35、CTAB、SDS.The hydrophobic interaction forces played a main role in the binding of Cyclen-Co（Ⅱ）with BSA.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第2期397-402,共6页
Journal of Sichuan University(Natural Science Edition)
基金
乐山市科技计划项目(14NZD021)
