期刊文献+

牛乳中嗜冷假单胞菌胞外蛋白酶的研究进展 被引量:3

Progress in Research on Extracellular Proteases Produced by Psychrotrophic Pseudomonas in Milk
下载PDF
导出
摘要 假单胞菌通常被认为是危害原料乳及其制品的主要嗜冷菌,其分泌的胞外蛋白酶具有耐热性,甚至能在超高温灭菌处理后仍有活性残留,并减短超高温灭菌乳的货架期。本文对来自牛乳中的嗜冷假单胞菌蛋白酶的研究进展做了总结,重点假单胞菌蛋白酶的催化机制研究进展,相关基因包括蛋白酶基因apr X在假单胞菌鉴别中的应用研究进展。最后讨论了嗜冷假单胞菌蛋白酶需要深入研究的方向。 Pseudomonas is highlighted as predominant psychrotrophic spoilage bacteria in milk and dairy products. Its extracellular proteases are heat-resistant, and have residual activity even after ultra high temperature sterilization(UHT) treatment leading to short life of UHT milk. The current article summarizes recent studies on extracellular proteases of psychrotrophic Pseudomonas in milk, with special emphasis on their catalytic mechanisms as well as the application of protease genes including apr X to identify Pseudomonas. At last, the further research directions in this area are discussed.
作者 徐煜 游春苹
出处 《乳业科学与技术》 2016年第1期24-27,共4页 JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
基金 "十二五"国家科技支撑计划项目(2012BAD12B08) 上海市科委优秀技术带头人计划项目(15XD1520300)
关键词 嗜冷菌 假单胞菌 蛋白酶 基因aprX psychrotrophic bacteria Pseudomonas protease aprX gene
  • 相关文献

参考文献35

  • 1MORITA R . Psychrophilic bacteria[J]. Bacteriological Reviews, 1975, 39(2): 144-167. 被引量:1
  • 2SORHAUG T, STEPANIAK L. Psychrotrophs and their enzymes in milk and dairy products: quality aspects[J]. Trends in Food Science & Technology, 1997, 8: 35-41. DOI:10,1016/S0924-2244(97)01006-6. 被引量:1
  • 3WIEDMANN M, WEILMEIER D, DINEEN S S, et al. Molecular and phenotypic characterization of Pseudomonas spp. isolated from milk[J]. Applied and Environmental Microbiology, 2000, 66(5): 2085- 2095. DOI: 10,1128/AEM.66.5,2085-2095,2000. 被引量:1
  • 4MARTINS M L, PINTO C L, ROCHA R B, et al. Genetic diversity of Gram-negative, proteolytic, psychrotrophic bacteria isolated from refrigerated raw milk[J]. International Journal of Food Microbiology, 2006, 111(2): 144-148. DOI:10,1016/j.ijfoodmicro,2006.06.020. 被引量:1
  • 5BAGLINIERE F, TANGUY G, JARDIN J, et al. Quantitative and qualitative variability of the caseinolytic potential of different strains of Pseudomonas fluorescens: implications for the stability of casein micelles of UHT milks during their storage[J]. Food Chemistry, 2012, 135(4): 2593-2603. DOI:10,1016/j.foodchem,2012.06.099. 被引量:1
  • 6HANTSIS-ZACHAROV E, HALPERN M. Culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits[J]. Applied and Environmental Microbiology, 2007, 73(22): 7162-7168. DOI: 10,1128/AEM.00866-07. 被引量:1
  • 7徐煜,任婧.原料乳中嗜冷假单胞菌危害及控制研究进展[J].食品工业科技,2015,36(13):380-384. 被引量:4
  • 8ENEROTH A, CHRISTIANSSON A, BRENDEHAUG J, et al. Critical contamination sites in the production line of pastreurised milk, with reference to the psychrotrophic spoilage flora[J]. International Dairy Journal,1998, 8: 829-834. DOI:10,1016/S0958-6946(98)00123-X. 被引量:1
  • 9de JONGHE V, COOREVITS A,van HOORDE K, et al. Influence of storage conditions on the growth of Pseudomonas species in refrigerated raw milk[J]. Applied and Environmental Microbiology, 201 I, 77(2): 460-470. DOI: 10,1128/AEM.00521-10. 被引量:1
  • 10MACHADO S G, da SILVA F L, BAZZOLLI D M, et al. Pseudomonas spp. and Serratia liquefaciens as predominant spoilers in cold raw milk[J]. Journal of Food Science, 2015, 80(8): M1842-M1849. DOI: 10,1111/1750-3841,12957. 被引量:1

二级参考文献77

  • 1任静,张兰威.原料乳中嗜冷菌的检测[J].食品工业科技,2007,28(3):217-221. 被引量:12
  • 2Champagne C P,Laing R R,Roy D,et al.Psychrotrophs i dairy products:their effects and their control [ J ].Critical Reviewt in Food Science and Nutrition,1994,34(1):1-30. 被引量:1
  • 3Hantsis-Zacharov E,Halpern M.Culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits [ J ].Applied and Environmental Microbiology,2007,73(22):7162-7168. 被引量:1
  • 4Samarzija D,Zamberlin S,Pogacic T.Psychrotrophic bacteria and their negative effects on milk and dairy products quality [ J ].Mljekarstvo,2012,62(2):77-95. 被引量:1
  • 5Magan N,Pavlou A,Chrysanthakis I.Milk-sense:a volatile sensing system recognises spoilage bacteria and yeasts in milk [ J].Sensors and Actuators B-Chemical,2001,72(1):28-34. 被引量:1
  • 6Pettipher G L,Mansell R,Mckinnon C H,et al.Rapid membrane filtration-epifluorescent microscopy technique for direct enumeration of bacteria in raw-milk [ J ].Applied and Environmental Microbiology,1980,39(2):423-429. 被引量:1
  • 7Manzano S,Antonio Ord6nez J,Delahoz L,et al.A rapid method for the estimation of the microbiological quality of refrigerated raw milk based on the aminopeptidase activity of gram-negative bacteria[ J ].International Dairy Journal,2005,15(1):79-84. 被引量:1
  • 8Firstenberg-Edenr,Tricarico M K.Impedimetric determination of total,mesophilic and psychrotrophic counts in raw milk [J].Journal of Food Science,1983,48(6):1750-1754. 被引量:1
  • 9Gutierrez R,Gonzalez I,Garcia T,et al.Monoclonal antibodies and an indirect ELISA for detection of psychrotrophic bacteria in refrigerated milk [ J ].Journal of Food Protection,1997,60(1):23-27. 被引量:1
  • 10Bovenizer J S,Jacobs M B,O'sullivan C K,et al.The detection of pseudomonas aeruginosa using the quartz crystal microbalance [ J].Analytical Letters,1998,31(8):1287-1295. 被引量:1

共引文献10

同被引文献24

引证文献3

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部