摘要
目的探讨应用高分辨磁共振（MR）血管壁成像技术评价兔腹主动脉粥样硬化模型斑块负荷的价值。方法30只新西兰白兔，通过高脂饮食联合内膜剥脱术建立腹主动脉粥样硬化模型。数字抽签随机分为1周组、1月组和2月组，分别于术后相应时间点进行兔腹主动脉血管壁的MR检查，随后24h内采集腹主动脉标本，进行组织学检查。统计学分析MR血管壁成像和组织学检查在测量管壁厚度、管壁面积等方面的一致性。结果30只新西兰白兔中，3只由于麻醉或手术过程中意外死亡，1只因血管条件较差造模未成功，在随后的饲养过程中有3只由于腹泻或感染等原因死亡，最终完成全部实验23只：1周组7只，1月组7只，2月组9只。1周、1月及2月各组实验动物组织学管壁面积测量值逐渐增大，分别为1.7663mm2、2．4371mm2和3．5978mm2，各组间比较差异有统计学意义（F=5．052，P=0．017）；由MR图像测量的血管壁面积、厚度和组织学测量值间呈线性相关性（r=0．688，P=0．001；r=0．642，P=0．002）。结论高分辨MR血管壁成像技术能够准确地评价并动态地观察病变早期斑块的负荷。
Objective To study the value of magnetic resonance vessel wall imaging in assessing the atherosclerotic plaque burden of rabbit model. Methods We built up abdominal atherosclerotic animal model in 30 New Zealand rabbits by high lipid diet combined with abdominal artery denudation. The animals were divided into 3 groups randomly, which were the 1-week group, 1-month group and 2-months group. The MRI and histology examination were carried out at relative time points. The correlations of area or thickness of vessel wall by MRI with histology examination were analyzed. Results Among the 30 rabbits, 3 died due to anesthesia or surgery, one rabbit model failed because of the thin vessel, and another 3 died of diarrhea or inflammation during the high lipid diet feeding. Eventually, totally 23 rabbits fulfilled the examinations （7 in 1-week group, 7 in I month group and 9 in 2-months group）. The vessel wall area of histology examination grew larger along with the posv surgery duration, from 1. 7663 mm2 of 1-week group to 2. 4371 mm2 of the 1-month group till 3. 5978 mm2 of 2-months group, with statistic significant difference among 3 groups （F= 5. 052,P=0. 017）. There were strong correlations of area or thickness vessel wall resulted from MRI with histology examination（r=0. 688, 0. 642; P=0. 001, 0. 002）. Conclusions High resolution MR vessel wall imaging technique may evaluate and follow up the plaque burden in the early stage of atherosclerosis.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2016年第3期312-316,共5页
Chinese Journal of Geriatrics
基金
国家自然科学基金（81101033）
关键词
动脉粥样硬化
磁共振成像
Atherosclerosis
Magnetic resonance imaging
