摘要
以五味子为原料水提多糖，经超滤、去蛋白后制得不同分子质量的五味子多糖（Schisandraceae polysaccharide，SP）组分SPl（分子质量100ku以上）和SP2（分子质量10～100ku），使用Caco-2和HT-29两种肠道肿瘤细胞检测体外抗肿瘤活性。结果表明：两种SP干预的肿瘤细胞增殖均受到抑制，实时荧光定量实时定量聚合酶链式反应检测发现Caspase-3相对表达量均出现了一定程度升高。其中SP1抑制作用较小，需较高剂量（500μg／mL以上）孵育较长时间（72h）才有显著抑制效果（P〈0．05），SP2抑制作用明显，显著抑制所需孵育时间和剂量都更少，对Caspase-3相对表达量提升更大。实验显示sP具有很好的抑制肠道肿瘤增殖潜力，小分子质量组分抗癌活性更强。
Polysaccharide components SP1 and SP2 with different molecular weights were obtained from Schisandraceae and through ultra-filtration and deproteinization. The intestinal cell lines Caco-2 and HT-29 were used to evaluate the antitumor activity of SP1 and SP2 in vitro. The results showed that the proliferation of both Caco-2 and HT-29 cells was inhibited by SP1 and SP2. Meanwhile, the increase in relative transcription level of caspase-3 was confirmed by real- time quantitative polymerase chain reaction （real time-qPCR）. SP1 had no significant effect on the inhibition of tumor cell growth, except incubation for 72 h at a dose of 500 pg/mL （P 〈 0.05）. However, SP2 showed an obvious tumor inhibitory effect even at lower dose and shortened incubation time, resulting in a more significant increase in the relative transcription level of caspase-3. In conclusion, the polysaccharides from Schisandraceae have great potential to inhibit the proliferation of intestinal tumor cells, especially the small molecular weight polysaccharide.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第5期192-196,共5页
Food Science
基金
哈尔滨市应用技术研究与开发项目(2013RFXXJ080)
关键词
五味子
多糖
抗肿瘤
实时定量聚合酶链式反应
Schisandraceae
polysaccharides
anti-tumor activity
real-time quantitative polymerase chain reaction
