摘要
以Os PT6转基因T5代大豆为材料,以其受体亲本东农50为对照,对低磷胁迫处理下的根系c DNA文库进行数字基因表达谱分析,共筛选得到33个差异表达基因。以受体亲本为对照,在Os PT6转基因大豆中上调表达的差异基因有21个、下调表达的差异基因有12个。GO功能显著性富集分析表明,有25个差异表达基因在蛋白、核酸等生物大分子代谢、次生代谢和酶活性调节等过程中表现为富集。KEGG代谢通路分析表明仅有1个过氧化物酶超蛋白家族基因参与到苯丙合成、苯丙氨酸代谢和次生代谢产物合成等次生代谢途径中。综合分析表明:通过光合作用相关酶类活性的调节控制光合作用速率从而影响次生代谢途径可能是大豆适应低磷胁迫的主要途径。以上结果为大豆响应低磷胁迫相关分子机制的深入研究和功能基因的筛选奠定了基础。
In this study,the T5 line of the soybean cuhivar transferred with OsPT6 gene and its receptor parent Dongnong 50 under low phosphorus stress were used to analyze their root cDNA library by digital gene expression profiling technology. The results showed that a total of 33 differential expressed genes（DEGs） were screened. Compared with the receptor parent, 21 DEGs were up-regulated and 12 DEGs were down-regulated in the OsPT6 transgenic soybean. GO enrichment analysis showed that 25 DEGs were enriched in the processes of biological macromolecules metabolism, secondary metabolism and enzyme activity regulation. The KEGG pathway analysis showed that only one peroxidase superfamily protein was involved in the pathways such as phenylpropanoid biosynthesis, phenylalanine metabolism and biosynthesis of secondary metabolites. Comprehensive analysis showed that the photosynthesis rate affected the secondary metabolic pathways by regulating activity of the enzymes involved in photosynthesis ,which could be the main way of soybean adapted to low phosphorus stress. These results provided a base for further study on the molecular mechanism in response to low phosphorus stress and functional genes screening in soybean.
出处
《大豆科学》
CAS
CSCD
北大核心
2016年第2期213-221,共9页
Soybean Science
基金
国家农作物转基因重大专项(2011ZX08004-005
2013ZX08004-005
2014ZX08004-005)
国家高技术研究发展计划"863计划"(2011AA10A105)
国家重点基础研究发展计划"973计划"(2011CB109301)
教育部长江学者和创新团队发展计划(PCSIRT13073)
江苏省现代作物生产协同创新中心(JCIC-MCP)
关键词
大豆
低磷胁迫
数字基因表达谱
差异表达基因
Soybean
Low phosphorus stress
Digital gene expression profiling
Differential expressed genes
