摘要
目的：探讨不同载体结合兔骨髓间充质干细胞（ bone marrow mesenchymal stem cells ， BMSCs）移植联合高压氧修复海水浸泡兔桡骨开放性骨缺损的成骨效果。方法48只新西兰大白兔，采用数字表法随机分为4组：单纯BMSCs移植＋高压氧组（ A组）、自体骨结合BMSCs移植＋高压氧组（ B组）、聚乳酸聚乙醇酸复合物（ PLGA）结合BMSCs移植＋高压氧组（ C组）、明胶海绵结合BMSCs移植＋高压氧组（ D组），每组12只。于兔双侧桡骨中段制作15 mm骨缺损，伤口浸泡于人工海水3 h，然后清创。移植于清创术后立即进行，术后进行高压氧治疗1 h，1次／d，连续2周。于伤后第4、8、12周分批处死后取材（每组每批4只兔，共8肢桡骨）。通过X线摄片、骨痂灰度值、苏木精－伊红（ HE）染色、生物力学检测，比较各组缺损区修复情况。结果（1）X线片观察，4组实验兔的成骨效果依次B组＞C组＞D组＞A组。（2）骨痂灰度值测量，伤后第12周时，各组骨缺损区平均骨痂灰度值：A组为162．1±1．3、B组为220．1±1．2、C组为195．6±1．7、D组为185．3±1．6，组间比较差异有统计学意义（ P＜0．05）。（3） HE 染色观察，兔伤后第4周时，C、D组可见材料残余，B组成骨质量最佳，C 组优于D组，D组优于A组，骨缺损区均可见骨性修复；伤后第12周时，C、D材料均降解，B组成骨质量最佳，C 组优于D组，D组优于A 组，基本是成熟骨组织。A、B、C、D组间比较差异有统计学意义（P＜0．05）。（4）生物力学检测，伤后第12周，A、B、C、D 4组每组8肢（n＝8），B组抗折载荷能力最强；A组最差，C组优于D组，组间比较差异具有统计学意义（P＜0．05）。结论（1）自体骨、PLGA、明胶海绵作为载体结合BMSCs配以高压氧疗法均可以修复骨缺损，比单独BMSCs移植＋高压氧的成骨质量更佳。自体骨组的成骨质量最�
Objective To investigate the osteogenic effect of different carriers applied with rabbit bone marrow mesenchymal stem cell （ BMSC ） transplantation combined with HBO in the treatment of open rabbit radial bone defect associated with seawater immersion .Methods Forty-eight New Zealand white rabbits were randomly divided into 4 groups：Group A （the simple BMSC transplantation ＋HBO group）, Group B （BMSC transplantation ＋Autologous bone ＋HBO group ） , Group C （ the BMSC transplantation ＋PLGA ＋HBO group） , and Group D （ the BMSC transplantation ＋gelatin sponge ＋HBO group ） , each consisting of 12＆amp;nbsp;animals.Bilateral bone defects with a length of 15mm were made in the middle of the radial bone , and then debridement was made 3 hours after immersion in the artificial seawater .Bone tissue transplantation was performed right after debridement , and HBO therapy was implemented 1 hour a day for a succession of 2 weeks. The animals were sacrificed 8 and 12 weeks after surgery in batches of 4 in each group , and a total of 8 radial bones were collected .The repair of bone defects in each group of animals was compared between the 4 groups, throughdetectionbyX-ray,bonecallusgreyvalue,HEstaining,andbiomechanicaltesting.Results （1）X-ray detection indicated that osteogenic efficacy of the 4 groups was group B 〉group C 〉group D 〉group A. （2） As shown in Figure 1, 12 weeks after injury, bone callus grey values for the 4 groups were respectively as follows：group A：162.1 ±1.3;group B：220.1 ±1.2; group C：195.6 ±1.7 and group D：185.3 ±1.6. Statistical significance could be noted , when comparisons were made between the groups （P〈0.05）.（3） HE staining indicated that residual material could be seen in group C and D , 4 weeks after injury .Bone repair could all be seen in the bone defect areas , with the bone repair of group B being the best , and the bone repair of group C was superior to that of group D , and the bone repair of group D was superior to that
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2015年第6期446-451,共6页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
基金
南京军区重点课题（11Z011）
关键词
海水浸泡
开放性骨缺损
骨髓间充质干细胞
高压氧
载体
兔
Seawater immersion
Open bone defect
Bone marrow mesenchymal stem cell
Hyperbaric oxygen
Carriers
Rabbits
