摘要
棉花黄萎病是棉花上的重要病害,严重威胁着棉花的产量.从长江流域4个省即湖北省、湖南省、江西省和安徽省采集300多个菌株,选择各省不同县市的67个菌株,对这67个供试菌株进行培养性状的鉴定、致病力的测定以及遗传多样性的分析,结果表明,在67个供试菌株中,就致病力类型而言,强致病力类型、中等致病力类型和弱致病力类型分别占35.82%、43.28%和17.91%.就培养性状类型而言,菌核型、中间型和菌丝型分别占38.81%、22.39%和38.81%.菌株的致病力和菌落培养类型均与地理位置没有相关性,但菌株的致病力与培养性状类型之间存在一定的相关性,强致病菌株大多数为菌核型,而弱致病菌株则以菌丝型为主.利用筛选出的15对RAPD引物对供试菌株DNA扩增并进行遗传多样性的分析结果表明,遗传多样性与菌株来源地无显著相关性,同时,菌株的遗传多样性与菌株致病力和培养性状也没有显著相关性.
Cotton Verticillium wilt is an important disease in the world,and a serious threat to cotton production. The four provinces,Hubei Province,Hunan Province,Jiangxi Province and Anhui Province,lie in the Yangtze River Basin. More than three hundreds isolates were collected and 67 isolates were selected from different cities and counties as the tested strains,then the cultural character,the pathogenic diversity and genetic diversity of the 67 strains were analyzed. The results showed that the high virulent types,the medium virulent types and the weak virulent types accounted for 35. 82%,43. 28% and 17. 91% respectively. The microsclerotia types,the intermediate types and the hyphae types acounted for 38. 81%,22. 39% and38. 81% respectively. Further analysis showed that there was no correlation between the pathogenicity,the cultural character and the strain origin. However,there were some correlations between the pathogenicity and the cultural character. Most of high virulent strains belonged to microsclerotia types and the weak virulent strains dominate hyphae types. Fifteen pairs of RAPD primers were gained for DNA amplification,cluster analysis showed that the genetic diversity had no significant correlation with the strain origin. Furthermore,the genetic diversity of the strains had no significant correlation with the pathogenicity and the cultural character.
出处
《湖北大学学报（自然科学版）》
CAS
2016年第2期120-126,134,共8页
Journal of Hubei University：Natural Science
基金
长江中游棉区棉花重大病虫害防控技术研究与集成示范项目(2012BAD19B05-006)资助
关键词
长江流域
棉花黄萎病菌
培养性状
致病力多样性
遗传多样性
the Yangtze River Basin
cotton Verticillium dahliae
culture character
the pathogenic diversity
the genetic diversity
