摘要
目的:建立临床有效方剂双莲方的全时段多波长融合指纹图谱,为双莲方的质量控制提供依据。方法:采用高效液相色谱法,Welch Ultimate LP-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相0.1%甲酸水(A)-甲醇(B)进行梯度洗脱(0~20 min,25%~60%A;20~40 min,60%~65%A%;40~70 min,65%~45%A),流速1.0 m L·min^(-1),柱温30℃,检测波长254,300,320,365 nm。结果:以木犀草素的色谱峰为参照峰,共确定18个色谱峰,建立了临床有效方剂双莲方的全时段多波长指纹图谱。根据此图谱,对其他成分进行相对定量,规定双莲方中其他成分相对木犀草素的含量不得低于0.39,0.24,0.46,0.45,0.73,0.28,0.16,0.41,0.51,2.37,0.77,0.22,0.60,0.36,0.62,0.80,0.52,0.96。结论:该方法简单、准确、科学,适用于双莲方的质量评价。
Objective： To establish the fingerprints of Shuanglianfang based on full-time multi-wavelength fusion method, and provide basis for the quality control of Shuanglianfang. Method： High performance liquid chromatography （HPLC） method was used on Welch Ultimate LP-C18 column （4.6 mm×250 mm,5 μm）, at the mobile phase of 0.1% methanoic acid solution （A） and ethanol （B） for gradient elution （0-20 min, 25%-60%A; 20-40 min, 60%-65%A;40-70 min, 65%-45%A）. The flow rate was maintained at 1.0 mL · min^-1 and the temperature was maintained at 30 ℃. Detector wavelengths were 254, 300, 320, 365 nm. Result： With the chromatographic peak of luteolin as the reference peak, 18 chromatographic peaks in total were identified, and the full-time multi-wavelength fusion fingerprints of Shuanglianfang were established. Based on the HPLC fingerprints, relative quantification of other components in Shuanglianfang was conducted, and the content of other components in Shuanglianfang shall not be less than 0.39, 0.24, 0.46, 0.45, 0.73, 0.28, 0.16, 0.41, 0.51, 2.37, 0.77, 0.22, 0.60, 0.36, 0.62, 0.80, 0.52, and 0.96 relative to luteolin. Conclusion： This method is simple, accurate, scientific, and suitable for the quality evaluation of Shuanglianfang.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第5期71-74,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"重大新药创制"科技重大专项(20132X09507005-004)
关键词
双莲方
指纹图谱
全时段多波长融合
木犀草素
Shuanglianfang
fingerprints
full time multi-wavelength fusion
luteolin
