摘要
目的探讨解整合素-金属蛋白酶33基因(ADAM33)3个位点(S2、ST+4、ST+5)单核苷酸多态性与新疆维吾尔族成人支气管哮喘的相关性。方法提取206例新疆维吾尔族成人支气管哮喘患者(哮喘组)与140例新疆维吾尔族成人健康体检者(对照组)的血液基因组DNA,采用限制性片段长度多态性方法分析ADAM33基因S2、ST+4、ST+5位点单核苷酸多态性,并对其中部分进行基因检测验证。结果两组ADAM33基因S2位点3种基因型分布差异有统计学意义(χ2=6.779,P=0.034),ST+4、ST+5位点3种基因型分布差异均无统计学意义(P>0.05)。ADAM33基因S2、ST+4、ST+5位点等位基因频率分布差异均无统计学意义(P>0.05);ADAM33基因ST+4与ST+5位点存在着弱连锁不平衡;哮喘组Hap4(CCT)显著低于对照组(χ2=4.761,P=0.029),Hap6(GAT)显著高于对照组(χ2=10.012,P=0.002)。结论 ADAM33基因S2、ST+4和ST+5位点单核苷酸多态性与新疆维吾尔族成人支气管哮喘具有相关性,在维吾尔族群体中ADAM33基因很可能存在有特异性易感的单体型或保护性单体型。
Objective To investigate the association of the S2,ST＋4,ST＋5locus in the ADAM33 gene polymorphism with bronchial asthma of Uygur adult population in Xinjiang.Methods Extract DNA from206 Uygur patients with bronchial asthma（asthma group）and 140 Uygur healthy individuals（control group）.PCR-RFLP was used to determine polymorphism of S2,ST＋4,ST＋5locus in ADAM33 gene,in which a part of the samples were chosen for genetic testing.Results The comparison of three genotypes of the S2 in the ADAM33 had statistical significance in asthma group and control group（χ^2=6.779,P =0.034）.But ST＋4and ST＋5had no statistical significance（P〉0.05）.The comparison of allelic frequencies of S2,ST＋4,ST＋5locus had no statistical significance（P〉0.05）.There was weak linkage disequilibrium between ST＋4and ST＋5in ADAM33gene;Linkage disequilibrium analysis showed that Hap4（CCT）in asthmatics was much lower than in controls（χ^2=4.761,P =0.029）,while Hap6（GAT）was much higher than in controls（χ^2=10.012,P =0.002）.Conclusion The Polymorphism of the S2,ST＋4,and ST＋5locus in the ADAM33 gene had significant correlation with bronchial asthma of Uygur population of adults in Xinjiang.The susceptible and protective haplotypes of asthmatics may exist in Uygur population.
出处
《新疆医科大学学报》
CAS
2016年第3期261-264,共4页
Journal of Xinjiang Medical University
基金
国家自然科学基金(81160004
81100026)
