摘要
目的:比较新型口腔生物陶瓷材料iRoot BP Plus和传统材料三氧化矿化聚合物(MTA)对LPS诱导小鼠炎症性骨破坏的影响。方法:24只BABL/C小鼠随机平分为4组,分别注射PBS、LPS(10 mg/kg)、iRoot BP Plus浸提液+LPS和MTA浸提液+LPS,1次/d。7d后取颅盖骨,通过显微CT扫描、HE染色、酶组织化学和组织免疫荧光染色,观察骨破坏程度、破骨细胞以及组织蛋白酶K的表达。结果:iRoot BP Plus和MTA可有效减轻小鼠颅盖骨炎性骨破坏,抑制破骨细胞的形成和吸收能力,并明显降低与破骨功能相关的组织蛋白酶K的表达。结论:iRoot BP Plus对小鼠炎性骨破坏有抑制作用。
Objective： To compare the effects of new oral bioceramic material iRoot BP Plus and mineral trioxide aggregate （MTA） on LPS--induced bone destruction in mice. Methods： 24 BABL/C mice were randomly equally di- vided into four groups injected by 10 mg/kg LPS with or without the material extracts or culture medium alone once a day. After treatment for 7 days, mice were sacrificed and calvarias were detected by micro-- computed tomo- graphy scanning, histologic analysis and double immunofluorescence staining. Results： iRoot BP Plus and MTA sig- nificantly alleviated bone destruction in mice calvarias, inhibited the formation and resorption capacity of osteo- clasts, and markedly downregulated the expression level of osteoclastogenic cathepsin K. Conclusion： iRoot BP Plus had inhibitory effects on inflammatory bone destruction in mice.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2016年第2期113-116,共4页
Journal of Oral Science Research
基金
国家自然科学基金资助项目(编号:81371131
81570973)
