摘要
以玉米(Zea mays L.)品种郑单958幼苗第2个新展开的叶片为试验材料,运用双向电泳(2-DE)和质谱分析等技术,确定高温胁迫下与s HSP26相关蛋白及其与叶绿体关系。质谱鉴定结果显示,胶上存在24个蛋白质差异点,属于14个蛋白质,归为6种功能类别,其中42%蛋白质参与光反应。与其对照相比,用s HSP26抗体(AT-s HSP26)处理后,与光合作用相关蛋白(ATP合酶α,β和γ亚基、放氧增强子蛋白(OEE1)、叶绿素a/b结合蛋白、细胞色素b6/f复合体铁硫亚基、光系统Ⅰ反应中心亚基Ⅳ和铁硫中心蛋白)表达下调。并且叶绿体PSⅡ的放氧速率明显降低。表明s HSP26通过调控光合作用相关蛋白,从而在一定程度上对叶绿体起到保护作用,最终影响玉米叶绿体的耐热性。
In this paper,the two-dimensional electrophoresis and mass spectrometry were used to determine the proteins related to CP-s HSP26 and its role in chloroplast endurance. The results show there are 24 different protein spots in 2-DE,which belong to 14 proteins and are classified into 6 functional categories by mass spectrum identification. Wherein,42% proteins are involved in the light-dependent reactions. After treatment with the s HSP26 antibody（ AT-s HSP26）,the expressions of proteins related to photosynthesis,including ATP synthase alpha,beta and gamma subunits,oxygen-evolving enhancer protein 1（ OEE1）,chlorophyll a / b binding protein,cytochrome b6 / f complex iron-sulfur subunit,photosystem I（ PSI） reaction center subunit IV and iron-sulfur center,were down-regulated. This study indicated that s HSP26 protected these proteins and played pivotal roles in plant photosynthesis.In addition,the addtion of AT-s HSP26 significantly reduced the oxygen evolution rate of PS？？ under heat stress,suggesting that s HSP26 can eventually improve the maize tolerance to heat stress.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2015年第6期729-736,共8页
Journal of Henan Agricultural University
基金
国家自然科学基金项目(31171470)
关键词
玉米
s
HSP26
叶绿体蛋白
双向电泳
高温胁迫
maize（Zea mays L.）
s HSP26
chloroplast protein
two-dimensional electrophoresis
heat stress
