摘要
以红小豆(Vigna angularis)品种龙小豆4号和天津红为材料,在盆栽条件下,以喷施蒸馏水+正常土壤水分为对照(CK),于幼苗期对红小豆进行淹水(5d)处理,开展淹水胁迫及预喷施脱落酸(Abscisic acid,ABA)对红小豆茎部生理指标及产量的影响研究,为红小豆抗涝栽培提供理论依据。结果表明,红小豆幼苗期淹水引起茎部膜脂过氧化产物(H202和MDA)、渗透调节物质(可溶性糖、可溶性蛋白及脯氨酸)含量升高,SOD、POD、CAT活性提高,淹水处理5d导致龙小豆4号、天津红产量分别较CK显著下降8.40%、9.91%。预喷施外源ABA具有缓解淹水胁迫的效应,能显著降低红小豆幼苗茎部MDA含量,显著提高茎部POD和CAT活性,进一步提高龙小豆4号茎部SOD活性,增加天津红茎部脯氨酸、可溶性蛋白、可溶性糖含量。预喷施ABA使淹水4 d的龙小豆4号和天津红产量分别显著提高6.95%和4.46%。综上所述,淹水胁迫引起红小豆茎部H202积累和膜脂过氧化加剧,诱导保护酶活性及渗透调节物质含量增加以抵御胁迫,但随淹水胁迫时间延长最终导致产量下降;外源ABA可在一定程度上提高保护酶活性和渗透调节物质含量,提高抗氧化能力,减缓淹水胁迫危害。
In order to provide theoretical basis for adzuki bean waterlogging resistance cultivation,this study investigated the effects of waterlogging stress and pre-spraying abscisic acid on stem physiological indexes and yield of adzuki bean by waterlogging for five days at seedling stage,using adzuki bean varieties Longxiaodou 4(LXD4)and Tianjinhong(TJH)under potted conditions,with spraying distilled water and normal soil moisture as control(CK).The results showed that the contents of H2O2,MDA,proline,soluble sugar,soluble protein and the activities of SOD,POD and CAT in the stem of adzuki bean were increased under waterlogging stress at seedling stage.Compared with CK,the yields of LXD 4 and TJH were significantly decreased by 8.40%and 9.91%after five days of waterlogging,respectively.Pre-straying of exogenous ABA could alleviate the waterlogging stress,significantly reduce the MDA content,significantly increase activities of POD and CAT in the stem,further increase the SOD activity in the stem of LXD4,and increase the content of proline,soluble protein and soluble sugar of TJH stem.Pre-straying ABA significantly increased the yield of LXD4 and TJH after four days of waterlogging by6.95%and 4.46%,respectively.In summary,waterlogging stress caused the accumulation of H2O2 and membrane lipid peroxidation in the stem of adzuki bean,induced the increase of protective enzyme activity and osmotic adjustment substance content to resist stress,and finally led to the decrease of yield.Exogenous ABA could increase the activity of protective enzymes and the content of osmotic adjustment substances,improve antioxidant capacity and alleviate the harm of waterlogging stress.
作者
李琬
李博
项洪涛
何宁
王曼力
刘佳
LI Wan;LI Bo;XIANG Hongtao;HE Ning;WANG Manli;LIU Jia(Institute of Crop Cultivation and Tillage,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;Institute of Plant Protection,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)
出处
《河南农业科学》
北大核心
2021年第7期48-56,共9页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金面上项目(31871576)
黑龙江省农业科学院农业科技创新跨越工程杂粮杂豆科技创新专项(HNK2019CX05-12)
黑龙江省农业科学院科研项目(2020YYYF029,2020FJZX047)
。
关键词
红小豆
幼苗
淹水胁迫
外源脱落酸
茎部生理
产量
Adzuki bean
Seedling
Waterlogging stress
Exogenous ABA
Stem physiology
Yield
