摘要
正交试验优化栗蓬抗菌成分的提取工艺,进一步对提取液进行分级醇沉及去除蛋白操作后,采用反相高效液相色谱(reversed-phase high-performance liquid chromatography,RP-HPLC)法对所提抗菌成分进行定性及联合定量测定。结果表明,在最优条件下,一次的提取率可达88.2%。采用Lichrospher C18色谱柱(150 mm×4.6 mm,5μm),以甲醇0.1%的磷酸水溶液为流动相,梯度洗脱程序,检测波长为280 nm,流速1 m L/min,柱温30℃可较好地测定栗蓬中没食子酸、原儿茶酸、槲皮素的含量。各成分质量浓度与峰面积在测定范围内均呈良好的线性关系(r>0.999),平均加样回收率(n=9)分别为98.9%、96.0%和97.1%,相对标准偏差(relative standard deviation,RSD)分别为2.36%、0.77%和4.73%。
Li Peng is the Chaetomium of chestnut （involucre of plant chestnut）, also called the involucre of chestnut, thorn shell of chestnut, or Sarg. of chestnut etc. In the areas rich in Chinese chestnut, large amounts of waste Li Peng was produced, besides a small amount of which can be used for firewood, the rest will be discarded to rot or burned; however, it is not only a waste of resources, but also cause serious environmental pollution. Reports showed that Li Peng could be used as a raw material to cultivate chestnut mushrooms, but it was only an accessory ingredient, and the main ingredient was cotton seed shell of chestnut tree, so Li Peng has yet to be fully used. In some areas, the phenomenon of hyphae overgrowth occurred in chestnut mushrooms cultivated by Li Peng, the reason was due to its antibacterial effect. The purpose of this paper was to establish a simple and low-cost technique for extraction of antibacterial components from Li Peng. At the same time, a high sensitive, good repeatable and stable, simple and rapid method was used to determine the antibacterial ingredients of Li Peng. This paper used the single factor experiment and the orthogonal experiment optimization for the extraction process of antibacterial components in Li Peng. Furthermore, polysaccharide in the samples was precipitated by ethanol and the qualitative and quantitative combined determination of antibacterial components were carried out using reversed-phase high-performance liquid chromatography （RP HPLC） method after protein removal. Peng （150 results showed that the best way to under 1：40 material liquid ×4.6 mm, 5μm）, methanol extract the antibacterial composition of the 60-mesh fine slag in Li 70 W ultrasonic reflux 20 rain. Using Lichrospher C18 column phosphoric acid solution as the mobile phase, the flow rate of 1 mL/ min, the detection wavelength at 280 nm, column temperature of 30 ℃ with the gradient elution program, the gallic acid, protocatechuic acid, and quercetin content in Li Peng could be bett
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2016年第1期23-29,共7页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
河北省科技厅科技支撑项目(13227308)
关键词
栗蓬
抗菌成分
提取
联合测定
Li Peng
antibacterial constituents
extraction
combined determination
