摘要
目的:通过观察小鼠自主活动和戊巴比妥钠阈上剂量所致睡眠时间,比较安神补脑液过膜前、过膜后的镇静安神作用,探讨可能的作用机制。方法:利用小鼠自主活动实验观察各样品的镇静作用,利用戊巴比妥钠所致小鼠睡眠实验观察各样品的安神作用。Fdlin法测定小鼠脑内蛋白质含量,二苯胺试剂显色法测定小鼠脑内DNA含量,Oricinol试剂显色法测定小鼠脑内RNA的含量。结果:过膜前、过膜后的安神补脑液都能减少小鼠自主活动次数,具有一定的镇静作用;过膜前安神补脑液能延长戊巴比妥钠所致小鼠的睡眠时间,具有一定的安神作用。除过膜前低剂量组外,其余各组脑内DNA含量与空白组比均明显增加,RNA含量则无明显差异。与空白组比较,过膜后高、低剂量组蛋白质含量明显增加。结论:过膜后的安神补脑液有镇静作用,但无催眠作用,并对脑组织中DNA和蛋白质合成有一定的促进作用。
Objective：Compare the sedative and hypnotic effects of the unfiltered and filtered Anshen Bunao Solutions through observing the rats independent activities and pentobarbital threshold dose of sleeping time to explore the possible mechanism. Methods ：Evaluate the sedative effects of the solutions by observing the independent activities. Evaluate the hypnotic effect of the solutions by the pentobarbital sodium threshold dose of sleeping time. The contents of protein, DNA and RNA were investigated by Lowry methods,the diphenylamine reagent method and Oricinol reagent method. Results：Both the unfiltered and filtered Anshenbunao solutions could reduce the spontaneous activities so they have the sedative effects.The unfiltered Anshen Bunao solutions could increase the sleeping time by Pentobarbital sodium,thus to identify its hypnotic effects.In addition to the low dose group unfiltered groups the DNA contents of the brain in the other group has obviously increased compare to the control groups.There is no difference in RNA contents of the brain among the groups. The protein contents of the filtered high and low dose groups increased obviously compare to the normal group.Conclusion：The filtered Anshen Bunao solution reserved the sedative effects but lost the hypnotic effects. Both of the unfiltered and filtered Anshen Bunao solutions can improve the combining of the DNA and protein.
出处
《黑龙江医药》
CAS
2015年第6期1189-1192,共4页
Heilongjiang Medicine journal
基金
中医药行业科研专项(编号:201507004)
国家重大新药创制专项(编号:2012ZX09501001-004)
关键词
安神补脑液
膜滤工艺
镇静催眠
自主活动
Anshen Bunao Solutions
Membrane filtration
Sedative and hypnotic effect
Spontaneous activities
