摘要
目的:检测慢性鼻窦炎伴鼻息肉患者外周血中Th9、Th17及Treg细胞的表达水平,初步探讨3种细胞对鼻息肉形成的影响。方法:收集46例慢性鼻窦炎伴鼻息肉患者的外周静脉血为鼻息肉组,另收集22例单纯鼻出血或鼻中隔偏曲患者外周静脉血作为对照组。1采用流式细胞术检测鼻息肉组和对照组患者外周静脉血中Th9、Th17及Treg细胞的表达率。2用SYBR Green I实时荧光定量PCR检测两组患者外周静脉血中Th9,Th17及Treg细胞相关转录因子IL-9mRNA,PU.1,IRF-4,RoRc及Foxp3的表达水平。3采用SPSS16.0统计软件,分析三种细胞及相关转录因子在鼻息肉组与对照组外周血中的差异性,并对鼻息肉组Th9与Th17及Th17与Treg之间的相关性进行分析。结果:1鼻息肉组外周静脉血中Th9、Th17细胞的表达率(1.29%±0.18%,4.03%±0.69%)明显高于对照组(0.45%±0.14%,1.35%±0.26%),而外周静脉血中的Treg细胞的表达率(2.98%±0.13%)明显低于对照组(5.44%±0.57%),差异均有统计学意义(P<0.05)。2鼻息肉组的转录因子IL-9mRNA,PU.1,IRF-4,RoRc的相对表达量明显高于对照组,而Foxp3的相对表达量低于对照组,差异均有统计学意义(P<0.05)。3鼻息肉患者外周静脉血中Th9与Th17细胞之间无明显相关性(P>0.05),Th17与Treg细胞之间呈负相关(r=-0.549,P<0.05)。结论:外周静脉血中高水平的Th9和Th17细胞对鼻息肉的形成有一定促进作用,而Treg细胞的较低表达可能进一步加剧鼻息肉的发生,且在鼻息肉的发病机制中存在Th17/Treg失衡,Th17/Treg细胞平衡在鼻息肉的发病机制中起着重要的免疫调节作用。
Objective：To investigate the expression levels of Th9, Thl7 and Treg cells in peripheral blood of patients with chronic rhinosinusitis with nasal polyps（CRSwNP）, and explore the role of Th9, Thl7 and Treg cells in the progression of CRSwNP. Method： Forty-six cases with CRSwNP served as an experimental group, while 22 cases with simple nasal bleeding or nasal septum deviation served as a control group. The peripheral blood of patients in both groups was collected and analyzed. Using flow cytometry （FCM） to detect the expression rates of Thg, Th17 and Treg cells in peripheral blood. Using qRT-PCR to detect the expression of relevant transcription factor of Th9, Thl7 and Treg cells （IL-9mRNA, PU. 1, IRF-4, RoRc, and Foxp3）. Using SPSS16.0 to analyse the differentiations and the revelance among these three cells. Result： The expression rates of Th9 and Thl7 cells in patients with CRSwNP （1.29 % ± 0.18 %, 4.03 % ± 0.69 %） was higher than the control group （0. 45%±0.14%, 1.35% ±0.26%）. But the expression rates of Treg cells in the experimental group （2.98 %±0.13 % ）was significantly lower than the control group（5.44 % ± 0.57 % ）. The differences were statisti- cally significant（P〈0.05）. The expression of revelant transcription factor （IL-9mRNA, PU. 1, IRF-4, RoRc） in NP group was also higher than the control group. The expression of Foxp3 in the control group was higher than NP, the differences both were statistically significant （P〈0.05）.The difference between Th9 and Thl7 in pa- tients with NP was not significant （P〈0.05）, and the negative correlation was found between Thl7 and Treg （r=-0. 549,P〈0.05）. Conclusion：The high expression level of Th9 and Thl7 cells might promote the develop- ment of NP, whereas the low expression level of Treg cells might further aggravate the occurrence of NP. The main function of the imbalance of Th17/Treg cells may be immune regulation in the pathogenesis of nasal polys.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2016年第4期277-281,共5页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
镇江市社会发展项目资助(No:SH2013053)
