摘要
目的探讨血清25-羟维生素D3[25-hydroxyvitamin D,25-(OH)D3]水平对手足口病(HFMD)患儿外周血辅助性T细胞17/调节性T细胞(Th17/Treg)相关细胞因子[白细胞介素(IL)-17、IL-6、IL-10和转化生长因子-β(TGF-β)]表达的影响,分析25-(OH)D3与各指标间的相关性。方法选取HFMD患儿142例,按照25-(OH)D3含量将其分为25(OH)D3正常组(>50 nmol/L)45例、25(OH)D3不足组(37.5~50 nmol/L)56例、25(OH)D3缺乏组(<37.5 nmol/L)41例。通过电化学发光法(ECLIA)检测血清25-(OH)D3含量,并通过流式细胞术(FCM)检测外周血Th17、Treg百分数,酶联免疫吸附测定法(ELISA)检测血清中IL-17、IL-6、IL-10、TGF-β水平。结果 25-(OH)D3正常组、25-(OH)D3不足组、25-(OH)D3缺乏组3组相比,外周血Th17百分数和Th17/Treg比值、血清IL-17、IL-6水平逐渐升高,而外周血Treg百分数和血清IL-10、TGF-β水平依次降低,两两之间的差异有统计学意义(均P<0.05)。Th17、IL-17、Th17/Treg、IL-6与25-(OH)D3均呈负相关(r=-0.465、-0.531、-0.476、-0.649,均P<0.05),Treg、IL-10、TGF-β与25-(OH)D3均呈正相关(r=0.379、0.501、0.449,均P<0.05)。结论维生素D可能通过调节手足口病患儿外周血Th17/Treg免疫平衡系统,影响手足口病的发生。
Objective To investigate the effect of serum 25-hydroxyvitamin D3 [25-( OH) D3 ]level on peripheral blood Th17 /Treg cell and their related cytolines ( IL-17,IL-6,IL-10,TGF-β) in children with hand-foot-mouth disease ( HFMD);and to analyze the correlations between 25-( OH) D3 and each index. Methods The 142 children with HFMD were divided into three groups,including normal group,insufficient group and scarce group based on serum 25-( OH) D3 level. Serum 25-( OH) D3 level was determined by electrochemiluminescence immunoassay ( ECLIA). Th17 and Treg percentage in peripheral blood were detected by flow cytometry ( FCM). The serum levels of IL-17,IL-6,IL-10 and TGF-β were detected by enzyme linked immunosorbent assay ( ELISA). Results The percentage of Th17 with CD4 + T cells in peripheral blood,the Th17 /Treg ratio,and the serum levels of IL-17 and IL-6 were significantly increased as the level of 25-( OH) D3 decreased. The percentage of Treg with CD4 + T cells in peripheral blood,the serum levels of IL-10 and TGF-β were reduced as the level of 25-( OH) D3 decreased. There were significant differences between each two groups ( all P < 0. 05). There were significantly negative correlations of 25-( OH) D3 with Th17,IL-17,Th17 /Treg and IL-6 ( r =-0. 465,-0. 531,-0. 476 and-0. 649,respectively,all P < 0. 05);and also significantly positive correlations with Treg,IL-10 and TGF-β( r = 0. 379,0. 501 and 0. 449,respectively, all P < 0. 05). Conclusion Vitamin D may affect the pathogenesis of HFMD by regulating the immune system balance of Th17 /Treg in peripheral blood of children.
作者
何媛
何巍巍
周小娟
梁晓君
王文娟
江永清
卢军
HE Yuan;HE Wei-wei;ZHOU Xiao-juan;LIANG Xiao-jun;WANG Wen-juan;JIANG Yong-qing;LU Jun(Clinical Laboratory,the Hospital Affiliated to JiujiangCollege,Jiujiang 332000,Jiangxi,China)
出处
《广东医学》
CAS
2019年第14期2009-2012,共4页
Guangdong Medical Journal
基金
江西省卫生计生委科技计划项目(编号:20185489)
