摘要
目的观察滋阴疏肝方对雌性中枢性性性早熟（CPP）大鼠下丘脑-垂体-性腺轴（HPGA）的作用，并探讨其对大鼠体质量的影响。方法选择雌性5日龄SD大鼠，按随机数字表法分为正常组、模型组、西药组、中药组，每组8只。采用皮下注射达那唑300μg复制CPP模型；制模后10d（15日龄时）西药组开始皮下注射亮丙瑞林100μg／kg，中药组灌胃滋阴疏肝方煎剂15mL／kg，正常组和模型组灌胃生理盐水15mL／kg，均每日1次，共16d。观察各组大鼠体质量的变化，肉眼观察大鼠阴门开启情况；阴道涂片法观察出现第1个发情期动物数；取卵巢、子宫称质量计算器官指数；制作病理切片观察子宫、卵巢组织形态学改变并计算子宫壁厚度和卵巢黄体生成数；检测血清中的黄体生成素（LH）水平和下丘脑促性腺激素释放激素（GnRH）mRNA的表达。结果中药组大鼠自25日龄（滋阴疏肝方灌胃10d后）后体质量明显低于模型组（g：64．6±5．2比71．7±4．7），并持续到实验结束（g：102．6±7．0比111．5±8．0），差异均有统计学意义（均P〈0．05）。与正常组比较，模型组3113龄时阴门开启数（只：4比0）、子宫系数（mg／kg：764±208比473±186）、卵巢系数（mg／kg：306±72比202±62）、子宫壁厚度（μm：319±61比207±78）、卵巢黄体生成数（个：5．0±1．3比0．0±0．0）和血清LH（U／L：8．95±2．54比3．67±0．66）、GnRHmRNA（2-△△：1．52±0．33比1．01±0．27）表达均明显增加（均P〈0．05）；西药组和中药组31日龄时阴门开启数（只：0和0）、子宫系数（mg／kg：138±52、532±218）、卵巢系数（mg／kg：100±44、222±79）、子宫壁厚度（μm：156±82、231±68）、卵巢黄体生成数（个：0．0±0．0、1．1±0．4）和血清LH（U／L：5．19±1．83、6．23±1．57）及下丘脑GnRHmRNA（2-△△：1．18±0．17、1．21±0．16）表达水�
Objective To study the effect of nourishing Yin and soothing liver decoction on hypothalamus- pituitary-gonad axis （HPGA） in female central precocious puberty （CPP） rats and its influence on body mass. Methods Female Sprague-Dawley （SD） rats 5 days old were randomly divided into normal, model, western medicine and Chinese medicine groups, each n=8. The rats were subcutaneously administered with danazol 3001,zg individually to set up CPP model; after modeling for 10 days （15 days old）, the rats in western medicine group were subcutaneously administered leuprorelin 100 tzg/kg, while the rats in Chinese medicine group received nourishing Yin and soothing liver decoction （15 mL/kg） by gastric perfusion and the rats in normal and model groups received normal saline （15 mL/kg） by gastric perfusion, once daily in all groups for 16 days. The changes of body mass in every group were observed, naked eye visual inspection was conducted for the number of vaginal opening; the number of first estrus appearance was observed by vaginal smear test. Their ovaries and uterus were collected and weighed to calculate organ coefficients. The pathological sections were made to observe the morphological manifestations of uterus and ovary and the thickness of uterus wall and number of ovary corpus luteum were calculated. The levels of luteotropic hormone （LH） in serum and mRNA expressions of gonadotropin releasing hormone （GnRH） from hypothalamus were measured. Results In the rats of Chinese medicine group 25 days old after taking the decoction for 10 days, their body masses were decreased obviously compared with those in the model groups （g： 64.6 ± 5.2 vs. 71.7 ± 4.7）, and the situation continued to the end of the experiment （g： 102.6 ± 7.0 vs. 111.5± 8.0）, these differences being statistically significant （all P 〈 0.05）. Compared with normal group, the number of vaginal opening （rats： 4 vs. 0）, uterus coefficients （mg/kg： 764 ± 208 vs. 473 ± 186）, ovaries coefficients �
出处
《中国中西医结合急救杂志》
CAS
北大核心
2016年第1期71-75,共5页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
湖北省自然科学基金青年基金（2013CFB466）
湖北省科技计划项目（鄂科技发计[2013]10）
关键词
滋阴疏肝方
雌性大鼠
中枢性性早熟
下丘脑-垂体-性腺轴
体质量
Nourishing Yin and soothing liver decoction
Female rats
Central precocious puberty
Hypothalamus-pituitary-gonad axis
Body mass
