摘要
目的观察盐酸小檗碱(Berberine,BER)对肠易激综合征(IBS)大鼠肠上皮紧密连接的影响,探讨其机制。方法将50只SD大鼠随机分为5组,每组10只。除对照组正常进食水外,其他4组采用醋酸灌肠法建立IBS模型,造模成功后予如下方法处理:BER组予盐酸BER(100 mg/kg)灌胃;氨基胍组予高度选择性一氧化氮合酶(NOs)阻断剂氨基胍(100 mg/kg)腹腔注射;BER+氨基胍组予BER(100 mg/kg)灌胃,同时氨基胍(100 mg/kg)腹腔注射;生理盐水组予3 ml生理盐水灌胃。造模后第7天比较5组大鼠腹部回撤反应(abnominal withdrawl reflex,AWR)评分(直结肠扩张试验)、束缚应激试验后排便次数、血浆D-乳酸水平、肠上皮紧密连接(tight junction,TJ)蛋白(Occludin、ZO-1)的表达。结果与对照组比较,BER组、BER+氨基胍组、氨基胍组、生理盐水组4组AWR评分升高,束缚应激后排便增多,D-乳酸水平升高,肠上皮TJ蛋白Occludin、ZO-1表达下降,差异有统计学意义(P<0.05)。BER组AWR评分比生理盐水组明显降低(P<0.05),氨基胍组、BER+氨基胍组AWR评分与生理盐水组比较,差异无统计学意义(P>0.05),BER组AWR评分比BER+氨基胍组明显降低(P<0.05);与生理盐水组比较,BER组和BER+氨基胍组排便次数减少,D-乳酸水平降低,肠上皮蛋白;Occludin、ZO-1表达升高,差异有统计学意义(P<0.05)。排便次数、D-乳酸水平、肠上皮TJ蛋白Occludin与ZO-1表达比较氨基胍组与生理盐水组差异均无统计学意义(P>0.05)。结论 BER影响IBS大鼠肠上皮紧密连接,其机制与一氧化氮有关。
Objective To observe the effect of Berberine （BER） on intestinal epithelium tight junction （TJ） in rats with irritable bowel syndrome （IBS） and to investigate its mechanisms. Methods The 50 SD rats were randomly divided into five groups. The control group took food and water normally （n = 10） , and IBS rat models were established by the acetic-acid clyster in other 4 groups. After successful model establishment, BER group was lavaged with BER solution （100 mg/kg, n = 10） ; Aminoguanidine group was given intraperitoneal injection with Aminoguanidine （100 mg/kg, n = 10） , which was a kind of blocking agent to nitric-oxide synthase （NOs） ; BER ＋ Aminoguanidine group was treated with BER solution intragastric administration （100 mg/kg） and simultaneous Aminoguanidine intraperitoneal injection （ 100 mg/kg, n = 10） ; saline group was lavaged with 3 ml physiological saline （n = 10）. Values of abdominal withdraw- al reflex （AWR） score, defecation frequency after restraint stress procedure, the plasma D-lactate level and expressions of TJ （Occludin and ZO-1） were compared in the five groups 7 d after model establishment. Results Compared with those in control group, the AWR scores, defecation frequency after restraint stress procedure and the plasma D-lactate level were significantly increased, while the expressions of TJ （ Occludin and ZO-1 ） were significantly decreased in BER, Aminoguanidine, BER ＋ Aminoguanidine and saline groups （P 〈 0.05 ）. Compared with that in saline group, AWR sco/e in BER group was significantly decreased （P 〈 0.05） , but the scores showed no statistically significant differences between Aminoguanidine and BER ＋ Aminoguanidine groups （ P 〉 0.05 ）. AWR score in BER group was significantly decreased compared with that in BER ＋ Aminoguanidine group （P 〈 0. 05）. Compared with those in saline group, the defecation frequency and plasma D-lactate level were significantly decreased, while the expressions of TJ Occludin
出处
《解放军医药杂志》
CAS
2016年第2期32-37,共6页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
南京军区医药卫生科研基金(12MA069)
漳州市自然科学基金(ZZ2013J29)
