摘要
副溶血性弧菌(Vibrio parahaemolyticus,Vp)是目前备受关注的人畜共患病的病原。为了比较7株不同来源的Vp对斑马鱼的毒力大小及致病能力,采用急性毒性试验测定96 h内Vp对斑马鱼的半数致死剂量(median lethal dosage,LD_(50)),用累积法和Bliss法对比分析LD_(50)指标。结果显示:Vp KNH1无致病性。其他菌株感染后,均表现出腹部出血,腹腔有腹水,内脏出血等症状。用累积法和Bliss法分析人类致病菌株ATCC17802、ATCC33847及水生动物致病菌株Vp13、Vp31、Vp41、Vp57共6株不同来源的副溶血性弧菌的LD_(50),累积法计算得到的LD_(50)分别为6.0×107、7.4×107、3.9×107、1.6×108、1.6×108、9.3×107CFU/m L;Bliss法得到的LD_(50)分别为:5.0×107、7.6×107、3.6×107、1.5×108、1.6×108、9.4×107CFU/m L。研究结果发现两种计算方法得出的LD_(50)数值差距较小,结果相似,不同菌株毒力强弱的顺序均为:Vp13>ATCC17802>ATCC33847>Vp57>Vp31≥Vp41>Vp KNH1。综上所述,累积法及Bliss法均可用于检测细菌毒力大小,但由于计算机的发展使得Bliss计算方法更加简便,为更优的选择。
The Vibrio parahaemolyticus（ Vp） is paid much attention to as a zoonosis pathogen at present. In order to compare the virulence and pathogenicity of seven Vp strains from different sources,we used zebrafish as the model to test median lethal dosage（ LD_（50）） by acute toxicity experiment within 96 h,and analyzed the data with Ree D-Muench method and Bliss method. The results suggested that the LD_（50） tested by Ree D-Muench method of human pathogenic bacteria（ ATCC17802,ATCC33847） and aquatic animal pathogens（ Vp13,Vp31,Vp41,Vp57） were 6. 0 × 107,7. 4 × 10~7,3. 9 × 10~7,1. 6 × 10~8,1. 6 × 108,9. 3 × 10~7 CFU / m L,respectively and 5. 0 × 10~7,7. 6 × 10~7,3. 6 × 107,1. 5 × 108,1. 6 × 10~8,9. 4 × 10~7 CFU / m L,respectively,with Bliss method. The results showed that these two kinds of calculation methods had little difference for the LD_（50）,and the virulence of these seven different Vp strains were as follows： Vp13 〉ATCC17802 〉ATCC33847〉 Vp57 〉Vp31〉 Vp41 〉Vp KNH1. To conclude,Ree D-Muench method and Bliss method can both be used to test Vp strains virulence. However,Bliss calculation method is more simple and accurate than the Ree D-Muench method with the development of computer technology. As a result,it is the better choice for LD_（50）.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2016年第1期86-96,共11页
Journal of Shanghai Ocean University
基金
上海市教育委员会科研创新项目(13ZZ127)
教育部留学回国人员科研启动基金资助项目(D-8002-15-0042)
上海海洋大学博士启动基金项目(A-0209-13-0105344)
水产动物遗传育种中心上海市协同创新中心项目(ZF1206)
