摘要
为了对防治朱顶红赤斑病提供理论基础,结合形态学与分子生物学的方法,对观赏性朱顶红赤斑病进行病原菌鉴定。结果表明:叶片接种赤斑病菌2d后开始发病,5d后叶片上产生赤色小点,后逐渐扩大为圆形或椭圆形病斑,发病症状与田间症状相似,从发病叶片上分离得到的病原菌分生抱子形态及大小均与初种菌株相同;病原菌ITS测序所得序列与NCBI对比,该病原菌菌株与Colletotrichum gloeosporioides strain HT31菌株的同源性为99%;LSU测序所得序列与NCBI对比,该病原菌菌株与Colletotrichum gloeosporioides strain CZ043C菌株的同源性为99%。结论:引起朱顶红赤斑病的病原菌为炭疽菌属胶孢炭疽菌(Colletotrichum gloeosporioides)。
To provide theoretical basis for prevention and control of amaryllis red spot disease,the methods of morphology and molecular biology were employed to identify the pathogenic bacteria.Results：After 2nd day of inoculation pathogen produced red leaf spot which changed into red spots after 5th day of inoculation and they regularly expanded to oval spots,these symptoms were similar to that in the fileds.The obtained pathogen conidia had shape and size similar to previous pathogen conidia;the obtained ITS sequence of concern pathogen had 99% similarity with the sequence obtained from NCBI and it was identical to Colletotrichum gloeosporioides strain HT31.LSU sequence was also compared with NCBI sequences,and the pathogen strains were related to C.gloeosporioides strain CZ043 C with 99%similarity.Conclusion：Amaryllis red spot disease was caused by C.gloeosporioides.
出处
《贵州农业科学》
CAS
2016年第1期72-74,共3页
Guizhou Agricultural Sciences
基金
国家自然科学基金(31560489)
关键词
朱顶红赤斑病
病原菌鉴定
amaryllis red spot disease
pathogenic identification
