摘要
目的 观察锯叶棕提取物（SR）对大鼠胶质瘤的作用及对其免疫系统的影响.方法 制作SD大鼠皮下胶质瘤模型,分成荷瘤组（n=10）、低剂量SR治疗组（n=10）、高剂量SR治疗组（n=10）,另取10只大鼠为空白对照组.大鼠造模后饲养1周开始给予锯叶棕提取物及安慰剂,低剂量组50 mg/kg、隔日灌胃一次,高剂量组300 mg/kg、隔日灌胃1次,其他组给予相同剂量的蒸馏水灌胃.喂养大鼠4周后,处死大鼠测瘤重、计算抑瘤率,TUNEL染色法检测肿瘤细胞凋亡,MTT法检测T淋巴细胞增殖活性.结果 （1）与荷瘤组比较,用药组瘤重明显减少（F =62.678,P=0.000）;与低剂量组比较,高剂量组抑瘤率明显提高（P＜0.05）.（2）荷瘤组肿瘤凋亡细胞明显偏少,用药组肿瘤凋亡细胞百分率明显增加,且SR高剂量组细胞凋亡最显著（F=1.287E3,P=0.000）.（3）与用药组及空白组比较,荷瘤组脾T淋巴细胞增殖活性降低;与荷瘤组比较,用药组T淋巴细胞增殖活性增加,且SR高剂量组T淋巴细胞增殖活性明显提高（F=103.565,P=0.000）.结论 锯叶棕提取物能够激活T淋巴细胞,增强机体免疫功能,杀伤肿瘤细胞、诱导肿瘤细胞凋亡,从而抑制了胶质瘤的生长,药物浓度越大、其抑瘤作用越明显.
Objective To investigate the effect of saw palmetto extract （SR） on GL261 glioma in rats and immune system.Methods The 40 rats were divided into 4 groups randomly,one was the control group without tumor （n =10）,the other 30 rats were given subcutaneous inoculation of tumor cells and then divided into 3 groups：tumor-bearing group （n =10）,low dose SR group （n =10）,and high dose SR group （n =10）.After 1 weeks feeded,the rats of SR groups were given the saw palmetto extract,low dose group 50 mg/kg once a day every other day and 300 mg/kg of high dose group every other day.The tumor-bearing groups received the same dose of distilled water.After 4 weeks feeding,we measured the tumor weight and the inhibition rate was calculated.The tumor cell apoptosis was detected by TdT mediated dUTP nick-end labeling （TUNEL） staining.The splenic T lymphocyte proliferation was tested by methyl thiazolyl tetrazolium （MTF）.Results （1） The SR groups compared to tumor group,the tumor weight was significantly reduced （F =62.678,P =0.000）.The tumor inhibition rate was significantly higher in high dose group.（2） The apoptosis of tumor cells in tumor-bearing group was significantly less than SR groups and the apoptosis was significantly increased after treatment with SR,especially in high dose SR group （F =1.287E3,P =0.000）.（3） Compared to SR groups and control group,T lymphocyte proliferation of tumor-bearing group reduced obviously.After treated with SR,T lymphocyte proliferation significantly increased and was higher in high-dose group （F =103.565,P =0.000）.Conclusions Saw palmetto extract can activate T lymphocytes and induce apoptosis to tumor cells.Its function was related to SR concentration.
出处
《中国医师杂志》
CAS
2016年第1期52-55,共4页
Journal of Chinese Physician
基金
辽宁省自然科学基金（2013021078）
