摘要
目的:对陵水暗罗Polyalthia nemoralis枝、叶中的化学成分进行分离和鉴定,并筛选细胞毒活性。方法:以硅胶、大孔吸附树脂和Sephadex LH-20色谱柱进行分离纯化,根据理化性质和光谱数据鉴定化合物的结构。采用MMT法筛选细胞毒活性。结果:从乙醇提取物中分离鉴定了14个化合物,即丁香酸(1),3-甲氧基-4-羟基肉桂酸(2),香草酸(3),4-羟基苯甲酸(4),毛里求斯排草素(5),番荔枝宁(6),(+)-oblongine(7),(+)-N-甲基网叶番荔枝碱(8),赤藓醇(9),D-甘露醇(10),乙基-β-D-吡喃葡萄糖苷(11),(+)木兰花碱(12),千金藤宁碱(13),(2S,4R)-4-羟基-2-哌啶羧酸(14)。结论:14个化合物均为首次从本属植物中分离得到;MTT法测得化合物6和13对多种肿瘤细胞有生长抑制作用。
Objective： To investigate the chemical constituents of the branches and leaves of Polyalthia nemoralis. Method： The compounds were isolated and purified by silica gel, macroporous adsorption resin and Sephadex LH-20 column chromatographic methods. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data. Result： Fourteen compounds were isolated and identified as syringic acid （1） , 3-methoxy-4-hydroxycinnamic acid （2） , vanillic acid （3） , 4-hydroxy- benzoic acid （4）, mauritianin （5）, （ ＋ ）-xylopinidine（6）, （ ＋ ）-oblongine（7）, （ ＋ ）-tembetarine（8）, eythritol （9）, D-mannitol （10）, ethyl-β-D-glucopyranoside （ 11 ）, （ ＋ ） -magnoflorine （ 12 ）, stepharanine （ 13 ）, （ 2S, dR） -4-hydroxy-2-piperidine-carboxylic acid （14） , respectively. Conclusion： All the compounds were isolated from the genus Polyalthia for the first time; compounds 6 and 13 showed inhibitation activities against multi tumor cell lines.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第8期1024-1027,共4页
China Journal of Chinese Materia Medica
关键词
陵水暗罗
化学成分
季铵生物碱
细胞毒活性
Polyalthia nemoralis
chemical constituents
quaternary alkaloids
cytotoxic activitivity
