摘要
目的:研究姜黄素对骨关节炎软骨细胞氧化应激的影响。方法:体外培养骨关节炎患者膝关节软骨细胞和正常关节软骨细胞,并以姜黄素干预,检测培养液一氧化氮(NO)、丙二醛产生量(MDA),检测一氧化氮合成酶(NOS)、超氧化物歧化酶活性(SOD);Real-Time PCR和Western blot法检测细胞Nrf2 m RNA及其蛋白表达水平。采用荧光共聚焦显微镜观察Nrf2的核转位情况,分析姜黄素对炎症损伤细胞的干预作用。结果:对关节炎软骨细胞,经姜黄素干预后,SOD、MDA、NOS、NO均有显著性变化。与正常软骨细胞组比较,关节炎软骨细胞组Nrf2 m RNA及其蛋白表达水平下调,细胞核内Nrf2活性增强;经姜黄素干预组变化更明显。结论:关节炎软骨细胞存在氧化应激反应,姜黄素能部分纠正氧化应激,对软骨细胞具有保护作用,机制可能与诱导Nrf2核转位从而激活Nrf2-ARE信号通路有关。
Objective To observe the effects of curcumin on oxidative stress in chondrocytes in osteoarthritis（OA）. Methods Chondrocytes from articular cartilage with OA and from the normal articular cartilage were prepared and cultured in vitro. The chondrocytes were treated with / without curcumin. Then the NOand MDA content in the nutrient solution, and the NOS and superoxide dismutase（SOD） activity were measured.The m RNA and protein expression of Nrf2 were detected by real-time PCR and Western blot. Laser scanning confocal microscopy was used to detect Nrf2 nuclear translocation so as to investigate its intervention on inflammatory injury cells. Results After treating with curcumin, the SOD, MDA, NOS and NO of the OA chondrocytes group had differences significantly. Compared with the normal chondrocyte group, the m RNA and protein expression of Nrf2 of the OA chondrocytes group was down-regulated, the Nrf2 activity in the nucleus was enhanced. And the change in the group treated with curcumin was obvious. Conclusions There are oxidative stress responses in the presence of osteoarthritis chondrocytes. Curcumin has a protective effect on cartilage cells, whose mechanism is related to induced nuclear translocation of Nrf2 and activated Nrf2-ARE signaling pathway.
出处
《实用医学杂志》
CAS
北大核心
2016年第2期188-191,共4页
The Journal of Practical Medicine
基金
河北省医学科学研究重点课题(编号:20150666)
